Use of intact rat brain cells as a model to study regulation of muscarinic acetylcholine receptors

Journal Article · Mon Aug 12 04:00:00 EDT 1985 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(85)90463-1· OSTI ID:5416383

Lee, J H; El-Fakahany, E E

Intact rat brain cells were dissociated and used to study the regulation of muscarinic acetylcholine receptors upon exposure to muscarinic receptor agonists. Incubation of cells with carbamylcholine resulted in a time-dependent decrease in subsequent (/sup 3/H)N-methylscopolamine specific binding, an effect which reached a steady state after 3 hr at 37/sup 0/C. This effect of carbamylcholine was dependent on the concentration of the agonist in the incubation medium and was due to a reduction in the maximal binding capacity of the receptor with no decrease in the affinity of the remaining receptors. This preparation might be useful in future studies to elucidate the mechanisms underlying the regulation of muscarinic acetylcholine receptors in the central nervous system. 20 references, 3 tables.

• Research Organization: Univ. of Maryland School of Pharmacy, Baltimore
• OSTI ID: 5416383
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 37:6; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETYLCHOLINE
AFFINITY
ALKALOIDS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOLOGICAL MODELS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CHEMICAL BONDS
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ESTERS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANS
PARASYMPATHOMIMETICS
QUATERNARY COMPOUNDS
RATS
RECEPTORS
RODENTS
STEADY-STATE CONDITIONS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES