Monitoring of pulmonary endothelial enzyme function: an animal model for a simplified clinically applicable procedure

Journal Article · Fri Jan 01 04:00:00 EST 1988 · Anesthesiology; (United States)

DOI:https://doi.org/10.1097/00000542-198801000-00008· OSTI ID:5412206

Toivonen, H J; Makari, N; Catravas, J D

The authors present a simple and clinically applicable method for the serial monitoring of pulmonary microvascular enzyme function in vivo. This method requires the intravenous injection of trace amounts of a radiolabelled substrate and the collection of a single arterial blood sample. Simultaneous measurement of pulmonary blood flow, (e.g., by dye- or thermo-dilution) and the determination of blood hematocrit are also needed for the calculations. This method was compared to the multiple blood sample indicator dilution method in normal anesthesized rabbits. Both methods gave identical results for the metabolism of the synthetic, hemodynamically inactive tripeptide, /sup 3/H-benzoyl-Phe-Ala-Pro (/sup 3/H-BPAP), by pulmonary microvascular endothelial angiotensin converting enzyme. The parameters measured were: 1) substrate utilization, expressed linearly and logarithmically, and 2) the apparent first order reaction constant. The new method was also used for the simultaneous measurement of single pass, transpulmonary metabolism of /sup 3/H-BPAP by angiotensin converting enzyme and of 5'-adenosine monophosphate by 5'-nucleotidase in rabbits in vivo. The authors propose that similar enzyme kinetic measurements could be used in clinical studies to test their usefulness as an aid in the early diagnosis of incipient pulmonary endothelial dysfunction, e.g., adult respiratory distress syndrome.

• Research Organization: Medical College of Georgia, Augusta
• OSTI ID: 5412206
• Journal Information: Anesthesiology; (United States), Journal Name: Anesthesiology; (United States) Vol. 68:1; ISSN ANESA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
ANIMALS
BIOLOGICAL FUNCTIONS
BIOLOGICAL MODELS
BLOOD FLOW
BODY
ENDOTHELIUM
ENZYMES
ESTERASES
FUNCTIONS
HYDROLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LUNGS
MAMMALS
METABOLISM
NUCLEOTIDASES
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PHOSPHATASES
PROTEINS
RABBITS
RESPIRATORY SYSTEM
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES