Beta adrenergic receptors in human cavernous tissue
Beta adrenergic receptor binding was performed with /sup 125/I iodocyanopindolol on human cavernous tissue membrane fractions from normal tissue and transsexual procedures obtained postoperatively, as well as from postmortem sources. Isotherm binding studies on normal fresh tissues indicated that the receptor density was 9.1 fmoles/mg. with a KD of 23 pM. Tissue stored at room temperature for 4 to 6 hours, then at 4C in saline solution for 19 to 20 hours before freezing showed no significant changes in receptor density or affinity, and provided evidence for the stability of postmortem tissue obtained within the same time period. Beta receptor density of 2 cavernous preparations from transsexual procedures was not significantly different from normal control tissues, and showed that high concentrations of estrogen received by these patients had no effect on beta adrenergic receptor density. Displacement of /sup 125/iodocyanopindolol by 5 beta adrenergic agents demonstrated that 1-propranolol had the greatest affinity followed by ICI 118,551, zinterol, metoprolol and practolol. When the results of these displacement studies were subjected to Scatfit, non- linear regression line analysis, a single binding site was described. Based on the relative potency of the selective beta adrenergic agents it appears that these receptors were of the beta 2 subtype.
- Research Organization:
- Wayne State Univ. School of Medicine, Detroit, MI
- OSTI ID:
- 5411241
- Journal Information:
- J. Urol.; (United States), Journal Name: J. Urol.; (United States) Vol. 133:4; ISSN JOURA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
AUTOPSY
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CHEMICAL BONDS
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ESTROGENS
HALOGEN COMPOUNDS
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE COMPOUNDS
IODINE ISOTOPES
ISOTHERMS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MAN
MATHEMATICS
NUCLEI
ODD-EVEN NUCLEI
PRIMATES
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
REGRESSION ANALYSIS
STABILITY
STATISTICS
STEROID HORMONES
SYMPATHOLYTICS
TRACER TECHNIQUES
VERTEBRATES