Modulation of ( sup 3 H)-glutamate binding by serotonin in the rat hippocampus: An autoradiographic study

Journal Article · Mon Dec 31 23:00:00 EST 1990 · Life Sciences; (United States)

DOI:https://doi.org/10.1016/0024-3205(91)90015-4· OSTI ID:5410622

Mennini, T; Miari, A ^[1]

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1. Istituto do Ricerche Farmacologiche Mario Negri, Milan (Italy)

Serotonin (5-HT) added in vitro increased ({sup 3}H)-glutamate specific binding in the rat hippocampus, reaching statistical significance in layers rich in N-Methyl-D-Aspartate sensitive glutamate receptors. This effect was explained by a significant increase in the apparent affinity of ({sup 3}H)-glutamate when 5-HT is added in vitro. Two days after lesion of serotonergic afferents to the hippocampus with 5,7- Dihydroxytryptamine ({sup 3}H)-glutamate binding was significantly decreased in the CA3 region and stratum lacunosum moleculare of the hippocampus, this reduction being reversed by in vitro addition of 10 {mu}M 5-HT. The decrease observed is due to a significant reduction of quisqualate-insensitive (radiatum CA3) and kainate receptors (strata oriens, radiatum, pyramidal of CA3). Five days after lesion ({sup 3}H)-glutamate binding increased significantly in the CA3 region of the hippocampus but was not different from sham animals in the other hippocampal layers. Two weeks after lesion ({sup 3}H)-glutamate binding to quisqualate-insensitive receptors was increased in all the hippocampal layers, while kainate and quisqualate-sensitive receptors were not affected. These data are consistent with the possibility that 5-HT is a direct positive modulator of glutamate receptor subtypes.

• OSTI ID: 5410622
• Journal Information: Life Sciences; (United States), Journal Name: Life Sciences; (United States) Vol. 49:4; ISSN 0024-3205; ISSN LIFSA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
AFFINITY
AMINES
AMINO ACIDS
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AUTORADIOGRAPHY
AZAARENES
AZOLES
BIOCHEMICAL REACTION KINETICS
BODY
BRAIN
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
DRUGS
GLUTAMIC ACID
HETEROCYCLIC COMPOUNDS
HIPPOCAMPUS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
INDOLES
KINETICS
MAMMALS
MEMBRANE PROTEINS
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
PYRROLES
RADIOPROTECTIVE SUBSTANCES
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SEROTONIN
SYMPATHOMIMETICS
TRITIUM COMPOUNDS
TRYPTAMINES
VERTEBRATES