Presynaptic localization of histamine H3-receptors in rat brain

Journal Article · Fri Jun 28 04:00:00 EDT 1991 · Biochemical and Biophysical Research Communications; (United States)

DOI:https://doi.org/10.1016/0006-291X(91)90624-G· OSTI ID:5410338

Fujimoto, K; Mizuguchi, H; Fukui, H; Wada, H ^[1]

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1. Osaka Univ. (Japan)

The localization of histamine H3-receptors in subcellular fractions from the rat brain was examined in a (3H) (R) alpha-methylhistamine binding assay and compared with those of histamine H1- and adrenaline alpha 1- and alpha 2-receptors. Major (3H)(R) alpha-methylhistamine binding sites with increased specific activities ((3H)ligand binding vs. protein amount) were recovered from the P2 fraction by differential centrifugation. Minor (3H)(R)alpha-methylhistamine binding sites with increased specific activities were also detected in the P3 fraction. Further subfractionation of the P2 fraction by discontinuous sucrose density gradient centrifugation showed major recoveries of (3H)(R)alpha-methylhistamine binding in myelin (MYE) and synaptic plasma membrane (SPM) fractions. A further increase in specific activity was observed in the MYE fraction, but the SPM fraction showed no significant increase in specific activity. Adrenaline alpha 2-receptors, the pre-synaptic autoreceptors, in a (3H) yohimbine binding assay showed distribution patterns similar to histamine H3-receptors. On the other hand, post-synaptic histamine H1- and adrenaline alpha 1-receptors were closely localized and distributed mainly in the SPM fraction with increased specific activity. Only a negligible amount was recovered in the MYE fraction, unlike the histamine H3- and adrenaline alpha 2-receptors.

• OSTI ID: 5410338
• Journal Information: Biochemical and Biophysical Research Communications; (United States), Journal Name: Biochemical and Biophysical Research Communications; (United States) Vol. 177:3; ISSN BBRCA; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ADRENALINE
AMINES
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZOLES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL LOCALIZATION
BODY
BRAIN
CARDIOTONICS
CARDIOVASCULAR AGENTS
CELL CONSTITUENTS
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM
CENTRIFUGATION
COMPARATIVE EVALUATIONS
DISTRIBUTION
DRUGS
EVALUATION
HETEROCYCLIC COMPOUNDS
HISTAMINE
HORMONES
HYDROGEN COMPOUNDS
IMIDAZOLES
ISOTOPE APPLICATIONS
KINETICS
LIGANDS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
RADIOASSAY
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SEPARATION PROCESSES
SUBCELLULAR DISTRIBUTION
SYMPATHOMIMETICS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ULTRACENTRIFUGATION
VERTEBRATES