Ascorbic acid reduces accumulation of (/sup 3/H)spiperone in mouse striatum in vivo
Journal Article
·
· Proc. Soc. Exp. Biol. Med.; (United States)
(/sup 3/H)Spiperone was administered to mice. In agreement with other published reports, 2 hr later the accumulation of tritium was three to four times greater in the corpus striatum than in the cerebellum. Ascorbic acid (100, 1000, 2000 mg/kg, ip, 30 min) reduced the 2-hr accumulation in the corpus striatum 16, 42, and 63%, respectively, with only the highest does producing any significant reduction in the cerebellum. The effect was still evident in striatum 18 hr after a single dose of 1000 mg/kg. Striatal minces taken from mice treated 1 or 2 hr earlier with ascorbic acid showed no reduction in (/sup 3/H)spiperone binding. However, preincubation of striatal minces for 2 hr with ascorbic acid (10/sup -3/ M) produced an 82% reduction in specific binding while not having any effect on nonspecific binding. While it cannot be certain that the reduction of striatal (/sup 3/H) spiperone concentrations after ascorbic acid in vivo was not a result of some nonspecific alteration in the pharmacokinetics of (/sup 3/H)spiperone, the in vitro observation strongly suggests that it resulted from an alteration of binding characteristics at the receptor level.
- Research Organization:
- Baylor College of Dentistry, Dallas, TX (USA)
- OSTI ID:
- 5409899
- Journal Information:
- Proc. Soc. Exp. Biol. Med.; (United States), Journal Name: Proc. Soc. Exp. Biol. Med.; (United States) Vol. 186:1; ISSN PSEBA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIDEPRESSANTS
ASCORBIC ACID
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL ACCUMULATION
BIOLOGICAL FUNCTIONS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CENTRAL NERVOUS SYSTEM AGENTS
CEREBELLUM
DRUGS
FUNCTIONS
IN VIVO
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MICE
NERVOUS SYSTEM
ORGANS
PSYCHOTROPIC DRUGS
REACTION KINETICS
RODENTS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
VITAMINS
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIDEPRESSANTS
ASCORBIC ACID
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL ACCUMULATION
BIOLOGICAL FUNCTIONS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CENTRAL NERVOUS SYSTEM AGENTS
CEREBELLUM
DRUGS
FUNCTIONS
IN VIVO
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MICE
NERVOUS SYSTEM
ORGANS
PSYCHOTROPIC DRUGS
REACTION KINETICS
RODENTS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
VITAMINS