Chromatographic band profiles and band separation of enantiomers at high concentration

Journal Article · Wed Aug 29 04:00:00 EDT 1990 · Journal of the American Chemical Society; (United States)

DOI:https://doi.org/10.1021/ja00174a007· OSTI ID:5407906

Jacobson, S; Golshan-Shirazi, S; Guiochon, G ^[1]

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1. Univ. of Tennessee, Knoxville (USA) Oak Ridge National Lab., TN (USA)

Experimental band profiles for large sample sizes of both single compounds and various binary mixtures of the optical antipodes of N-benzoylalanine are obtained on immobilized bovine serum albumin (BSA). The two enantiomers are resolved on this stationary phase. In the case of mixtures, the individual elution profiles are acquired by continuous fraction collection, followed by chromatographic analysis of these fractions. The equilibrium isotherms of each of the two chiral isomers between the two phases of the chromatographic system are determined by frontal analysis on the same column. These isotherms can be accounted for with great precision by a model assuming the existence of two kinds of sites on the stationary phase. For each isomer, the adsorption on each type of site is well described by a Langmuir equation. The experimental profiles are nearly identical with the profiles calculated by the semi-ideal model of nonlinear chromatography, using the competitive Langmuir isotherms derived from the measured single-component isotherms.

• OSTI ID: 5407906
• Journal Information: Journal of the American Chemical Society; (United States), Journal Name: Journal of the American Chemical Society; (United States) Vol. 112:18; ISSN 0002-7863; ISSN JACSA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400105* -- Separation Procedures
ALANINES
AMINO ACIDS
BINARY MIXTURES
CALCULATION METHODS
CARBOXYLIC ACIDS
CHROMATOGRAPHY
DATA
DISPERSIONS
ENANTIOMORPHS
EXPERIMENTAL DATA
INFORMATION
ISOMERS
ISOTHERMS
LIQUID COLUMN CHROMATOGRAPHY
MIXTURES
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
SEPARATION PROCESSES