Passage of albumin from the middle ear to the inner ear in otitis media in the chinchilla

Journal Article · Sat Aug 01 04:00:00 EDT 1981 · Am. J. Otolaryngol.; (United States)

DOI:https://doi.org/10.1016/S0196-0709(81)80017-8· OSTI ID:5406850

Goldberg, B; Goycoolea, M V; Schleivert, P M; Shea, D; Schachern, P; Paparella, M M; Carpenter, A M

A study of the permeability of the middle ear-inner ear interface for macromolecules was carried out in chinchillas with open and obstructed eustachian tubes utilizing tritiated human serum albumin and immunoelectrophoresis. Tritiated albumin was placed in the round window niche area or normal animals and animals in which the eustachian tubes had been obstructed for 24 hours or 14 days. The tritiated albumin was allowed to remain in the middle ear cavity for 24 hours, Samples of middle ear effusion, perilymph, blood and cerebrospinal fluid were collected and measured for radioactivity. Radioactivity was demonstrated in the perilymph. Samples of middle ear effusions and perilymph were also studied by immunoelectrophoresis with goat antihuman albumin. Albumin placed in the round window niche of an experimental animal could be recovered unchanged in the perilymph. The results suggest a pathophysiologic explanation for the association of otitis media and sensorineural hearing loss or endolymphatic hydrops.

• OSTI ID: 5406850
• Journal Information: Am. J. Otolaryngol.; (United States), Journal Name: Am. J. Otolaryngol.; (United States) Vol. 2:3; ISSN AJOTD
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
AUDITORY ORGANS
BIOLOGICAL MATERIALS
BLOOD
BODY
BODY FLUIDS
CEREBROSPINAL FLUID
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LYMPH
MATERIALS
ORGANIC COMPOUNDS
ORGANS
PERMEABILITY
PROTEINS
SENSE ORGANS
TRITIUM COMPOUNDS