Identification of two integral membrane proteins of Plasmodium falciparum

Journal Article · Fri Jul 01 04:00:00 EDT 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.14.5195· OSTI ID:5405995

Smythe, J A; Coppel, R L; Brown, G V; Ramasamy, R; Kemp, D J; Anders, R F ^[1]

---

1. Walter and Eliza Hall Institute of Medical Research, Melbourne (Australia)

The authors describe the isolation and cloning of two integral membrane protein antigens of Plasmodium falciparum. The antigens were isolated by Triton X-114 temperature-dependent phase separation, electrophoretically transferred to nitrocellulose, and used to affinity-purify monospecific human antibodies. These antibodies were used to isolate the corresponding cDNA clones from a phage {lambda}gt11-Amp3 cDNA expression library. Clone Ag512 corresponds to a M{sub r} 55,000 merozoite rhoptry antigen, and clone Ag513 corresponds to a M{sub r} 45,000 merozoite surface antigen. Both proteins can be biosynthetically labeled with ({sup 3}H)glucosamine and ({sup 3}H)myristic acid, suggesting that they may be anchored in membranes via a glycosylphosphatidylinositol moiety. Similarities in the C-terminal sequences of the M{sub r} 45,000 merozoite surface antigen and the Trypanosoma brucei variant surface glycoproteins provides further evidence that this antigen has a glycosylphosphatidylinositol anchor.

• OSTI ID: 5405995
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 85:14; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMALS
ANTIGENS
BIOCHEMISTRY
CARBOHYDRATES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMISTRY
ELECTROPHORESIS
GLUCOPROTEINS
GLUCOSAMINE
GLYCOPROTEINS
HEXOSAMINES
HEXOSES
HYDROGEN COMPOUNDS
INVERTEBRATES
LABELLING
MEMBRANES
MICROORGANISMS
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PLASMODIUM
PROTEINS
PROTOZOA
SACCHARIDES
SPOROZOA
TETRADECANOIC ACID
TRITIUM COMPOUNDS
VACCINES