Oxidation of reduced pyridine nucleotides by peroxyacyl nitrates

Journal Article · Tue Jan 01 04:00:00 EST 1963 · Arch. Biochem. Biophys.; (United States)

DOI:https://doi.org/10.1016/0003-9861(63)90319-9· OSTI ID:5405666

Mudd, J B; Dugger, Jr, W M

TPNH and DPNH are oxidized by peroxyacyl nitrates. The oxidation products are TPN and DPN, respectively, as judged by the formation of cyanide complexes, and reaction with appropriate substrates in the presence of glucose-6-phosphate dehydrogenase and alcohol dehydrogenase. The rate of oxidation of reduced pyridine nucleotides is increased by raising the pH from 7.4 to 8.8 in Tris buffer, and by increasing the buffer molarity at any one pH. The oxidizing power of peroxyacyl nitrates in solution dissipates regardless of the presence of oxidizable compound, and the rate of dissipation is more rapid as the pH is raised. At pH 8.0, the half-life of the oxidizing power is approximately 4 min. The rate and amount of pyridine nucleotide oxidation is independent of the initial concentration of reduced pyridine nucleotide over the range 3.7 x 10/sup -5/-1.1 x 10/sup -1/ M at pH 8.0. PAN prevents the incorporation of acetate into long-chain fatty acids by spinach chloroplasts, but does not inhibit the incorporation into citrate and glutamate.

• Research Organization: Univ. of California, Riverside
• OSTI ID: 5405666
• Journal Information: Arch. Biochem. Biophys.; (United States), Journal Name: Arch. Biochem. Biophys.; (United States) Vol. 102:1; ISSN ABBIA
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AZINES
BIOCHEMICAL REACTION KINETICS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHLOROPLASTS
ESTERS
FOOD
HETEROCYCLIC COMPOUNDS
KINETICS
METABOLISM
NITRATES
NITRIC ACID ESTERS
NITROGEN COMPOUNDS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDATION
OXYGEN COMPOUNDS
PEROXYACETYL NITRATE
PH VALUE
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
PLANTS
PYRIDINES
REACTION KINETICS
SPINACH
SYNTHESIS
TOXICITY
VEGETABLES