Surface labeling of Pneumocystis carinii from in vitro culture

Journal Article · Sun Jan 01 00:00:00 EST 1989 · Journal of Protozoology, The; (USA)

DOI:https://doi.org/10.1111/j.1550-7408.1989.tb02702.x· OSTI ID:5401840

Radding, J A; Armstrong, M Y; Bogucki, M S; Richards, F F ^[1]

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1. Yale Univ. School of Medicine, New Haven, CT (USA)

Pneumocystis carinii is an opportunistic pathogen of man, carried as a commensal in healthy subjects. It frequently causes a fatal pneumonia in the immunosuppressed host. It is a major complication of HIV-1 infection in man (AIDS). Using surface radioiodination of rat-derived P. carinii trophozoites obtained from in vitro culture, a major surface glycoprotein (gp120) has been identified. The glycoprotein exhibits adherent behavior similar to that of the intact organism. Purification of gp120 by conventional methods was unsuccessful as the glycoprotein irreversibly bound to numerous column matrices. A combination of gel chromatography and hydroxyapatite chromatography in sodium dodecylsulfate was utilized to purify the glycoprotein. Some preliminary characterization of the glycoprotein is presented.

• OSTI ID: 5401840
• Journal Information: Journal of Protozoology, The; (USA), Vol. 36:1; ISSN 0022-3921
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLYCOPROTEINS
CHEMICAL COMPOSITION
PNEUMONIA
PATHOGENESIS
AIDS
AUTORADIOGRAPHY
CHROMATOGRAPHY
ELECTROPHORESIS
FRACTIONATION
IMMUNOSUPPRESSION
IODINE ISOTOPES
MOLECULAR WEIGHT
PATHOGENS
DISEASES
IMMUNE SYSTEM DISEASES
INFECTIOUS DISEASES
ISOTOPES
ORGANIC COMPOUNDS
PROTEINS
RESPIRATORY SYSTEM DISEASES
SEPARATION PROCESSES
VIRAL DISEASES
550901* - Pathology- Tracer Techniques
550201 - Biochemistry- Tracer Techniques