Identification and purification of a human immunoglobulin-enhancer-binding protein (NF-. kappa. B) that activates transcription from a human immunodeficiency virus type 1 promoter in vitro
Journal Article
·
· Proceedings of the National Academy of Sciences of the United States of America; (USA)
- Rockefeller Univ., New York, NY (USA)
The enhancer-binding factor NF-{kappa}B, which is found only in cells that transcribe immunoglobulin light chain genes, has been purified from nuclear extracts of Namalwa cells (human Burkitt lymphoma cells) by sequence-specific DNA affinity chromatography. The purified NF-{kappa}B has been identified as a 51-kDa polypeptide by UV-crosslinking analysis. Footprint and methylation-interference analyses have shown that purified NF-{kappa}B has a binding activity specific for the {kappa} light chain enhancer sequence. The purified factor activated in vitro transcription of the human immunodeficiency virus type I promoter by binding to an upstream NF-{kappa}B-binding site.
- OSTI ID:
- 5401544
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 85:13; ISSN 0027-8424; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AIDS VIRUS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CONSTITUENTS
CHEMICAL ACTIVATION
CHEMICAL REACTIONS
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
EXTREME ULTRAVIOLET RADIATION
GENE REPRESSORS
GLOBULINS
IMMUNOGLOBULINS
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
NUCLEOPROTEINS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PEPTIDES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLASMIDS
POLYMERIZATION
POLYPEPTIDES
PROTEINS
PURIFICATION
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
TRANSCRIPTION
ULTRAVIOLET RADIATION
VIRUSES
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AIDS VIRUS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CONSTITUENTS
CHEMICAL ACTIVATION
CHEMICAL REACTIONS
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
EXTREME ULTRAVIOLET RADIATION
GENE REPRESSORS
GLOBULINS
IMMUNOGLOBULINS
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
NUCLEOPROTEINS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PEPTIDES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLASMIDS
POLYMERIZATION
POLYPEPTIDES
PROTEINS
PURIFICATION
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
TRANSCRIPTION
ULTRAVIOLET RADIATION
VIRUSES