Structure of the major carbohydrate fragment of the Leishmania donovani lipophosphoglycan
The major carbohydrate fragment from the lipophosphoglycan of Leishmania donovani was generated by mild acid hydrolysis (0.02 N HCl, 5 min, 100/sup 0/C) and purified by chromatography on DE-52 cellulose and thin layer. By a combination of analyses including gas-liquid chromatography-mass spectrometry and /sup 1/H NMR, the structure of the fragment was elucidated as PO/sub 4/..-->..6Gal(..beta..1-..-->..4)Man. Approximately 16 of these phosphorylated disaccharide units occur in the overall glycoconjugate structure. NMR analysis of an alkaline phosphatase treated phosphorylated tetrasaccharide generated from lipophosphoglycan showed that the phosphorylated disaccharide units are linked together via ..cap alpha..-glycosidic linkages. Complete characterization of the phosphorylated disaccharide units of lipophosphoglycan provides the first example of a defined carbohydrate anchored in membranes by a derivative of phosphatidylinositol.
- Research Organization:
- Univ. of Kentucky Medical Center, Lexington
- OSTI ID:
- 5400329
- Journal Information:
- Biochemistry; (United States), Vol. 26:19
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
DISACCHARIDES
NUCLEAR MAGNETIC RESONANCE
STRUCTURAL CHEMICAL ANALYSIS
PROTOZOA
BIOCHEMISTRY
ACID HYDROLYSIS
GAS CHROMATOGRAPHY
GLYCOLIPIDS
HEAVY WATER
MASS SPECTROSCOPY
NMR SPECTRA
PARASITES
PHOSPHOLIPIDS
PROTONS
PURIFICATION
THIN-LAYER CHROMATOGRAPHY
TRITIUM COMPOUNDS
ANIMALS
BARYONS
CARBOHYDRATES
CHEMICAL REACTIONS
CHEMISTRY
CHROMATOGRAPHY
DECOMPOSITION
ELEMENTARY PARTICLES
ESTERS
FERMIONS
HADRONS
HYDROGEN COMPOUNDS
HYDROLYSIS
INVERTEBRATES
LABELLED COMPOUNDS
LIPIDS
LYSIS
MAGNETIC RESONANCE
MICROORGANISMS
NUCLEONS
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
OXYGEN COMPOUNDS
RESONANCE
SACCHARIDES
SEPARATION PROCESSES
SOLVOLYSIS
SPECTRA
SPECTROSCOPY
WATER
550601* - Medicine- Unsealed Radionuclides in Diagnostics
550201 - Biochemistry- Tracer Techniques