Structure of the major carbohydrate fragment of the Leishmania donovani lipophosphoglycan
The major carbohydrate fragment from the lipophosphoglycan of Leishmania donovani was generated by mild acid hydrolysis (0.02 N HCl, 5 min, 100/sup 0/C) and purified by chromatography on DE-52 cellulose and thin layer. By a combination of analyses including gas-liquid chromatography-mass spectrometry and /sup 1/H NMR, the structure of the fragment was elucidated as PO/sub 4/..-->..6Gal(..beta..1-..-->..4)Man. Approximately 16 of these phosphorylated disaccharide units occur in the overall glycoconjugate structure. NMR analysis of an alkaline phosphatase treated phosphorylated tetrasaccharide generated from lipophosphoglycan showed that the phosphorylated disaccharide units are linked together via ..cap alpha..-glycosidic linkages. Complete characterization of the phosphorylated disaccharide units of lipophosphoglycan provides the first example of a defined carbohydrate anchored in membranes by a derivative of phosphatidylinositol.
- Research Organization:
- Univ. of Kentucky Medical Center, Lexington
- OSTI ID:
- 5400329
- Journal Information:
- Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 26:19; ISSN BICHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ACID HYDROLYSIS
ANIMALS
BARYONS
BIOCHEMISTRY
CARBOHYDRATES
CHEMICAL REACTIONS
CHEMISTRY
CHROMATOGRAPHY
DECOMPOSITION
DISACCHARIDES
ELEMENTARY PARTICLES
ESTERS
FERMIONS
GAS CHROMATOGRAPHY
GLYCOLIPIDS
HADRONS
HEAVY WATER
HYDROGEN COMPOUNDS
HYDROLYSIS
INVERTEBRATES
LABELLED COMPOUNDS
LIPIDS
LYSIS
MAGNETIC RESONANCE
MASS SPECTROSCOPY
MICROORGANISMS
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
NUCLEONS
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
OXYGEN COMPOUNDS
PARASITES
PHOSPHOLIPIDS
PROTONS
PROTOZOA
PURIFICATION
RESONANCE
SACCHARIDES
SEPARATION PROCESSES
SOLVOLYSIS
SPECTRA
SPECTROSCOPY
STRUCTURAL CHEMICAL ANALYSIS
THIN-LAYER CHROMATOGRAPHY
TRITIUM COMPOUNDS
WATER