Pressure effects on dipalmitoylphosphatidylcholine bilayers measured by sub 2 H nuclear magnetic resonance

Journal Article · Tue Apr 02 00:00:00 EST 1991 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00227a022· OSTI ID:5399185

Driscoll, D A; Samarasinghe, S; Adamy, S; Jonas, J; Jonas, A ^[1]

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1. Univ. of Illinois, Urbana (USA)

The effects of pressure, up to 5 kbar, on multilamellar vesicles of 1,2-dipalmitoyl-sn-phosphatidylcholine perdeuterated in the acyl chains (DPPC-d{sub 62}) were examined by using high-pressure NMR techniques. A deuterium probe was built, and the quadrupole splitting was measured against pressure at various temperatures. The experiments were performed on pure lipid bilayers in the liquid-crystalline state and on bilayers in the liquid-crystalline state containing the local anesthetic tetracaine. The results show that the order parameter of all segments of the acyl chains increases with pressure in the liquid-crystalline state. The more highly ordered regions of the chains are affected slightly more than the regions near the methyl ends. The addition of tetracaine increases the disorder of the chains, and pressure reverses the effect of anesthetic on the lipid as seen by the reversal of the changes in line shape and the measured order parameter.

• OSTI ID: 5399185
• Journal Information: Biochemistry; (United States), Vol. 30:13; ISSN 0006-2960
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANESTHETICS
BIOLOGICAL EFFECTS
MEMBRANES
PRESSURE EFFECTS
PHOSPHOLIPIDS
NUCLEAR MAGNETIC RESONANCE
DEUTERIUM COMPOUNDS
PHASE STUDIES
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DRUGS
ESTERS
HYDROGEN COMPOUNDS
LIPIDS
MAGNETIC RESONANCE
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
RESONANCE
550201* - Biochemistry- Tracer Techniques