Effects of electroconvulsive shock on noradrenergic and serotonergic receptors and their associated second messenger systems

Thesis/Dissertation · Sat Dec 31 23:00:00 EST 1988

OSTI ID:5398581

Blendy, J A

This thesis confirmed previous findings that repeated administration of electroconvulsive shock (ECS) increases alpha-1 adrenoceptors labeled by {sup 3}H-prazosin in rat fronto-parietal cortex. On the other hand, {sup 3}-WB4101 binding was unaffected by ECS treatment. {sup 3}H-prazosin labels two subtypes of alpha-1 adrenergic receptors with equal affinity. These subtypes are discriminated in the basis of their affinities for the antagonist WB4101. The subtype with high affinity for WB4101 has been termed alpha-1a, while that with lower affinity has been termed alpha-1b. Hence, when 60 nM prazosin is used to define specific binding, {sup 3} H-WB4101 labels primarily the alpha-1a adrenoceptor. When WB4101 was included in {sup 3}H-prazosin binding experiments at concentrations which mask the alpha-1a adrenoceptor, the fractional increase in binding caused by ESC was significantly higher. These findings indicate that ECS selectively increases the alpha-1b adrenoceptor subtype in rat cortex. Similarly, chronic administration of reserpine appears to increase predominantly the alpha-1b adrenoceptor since this treatment increases {sup 3} H-prazosin, but not {sup 3}H-WB4101 binding in rat frontoparietal cortex. Quantitative autoradiography confirms and extends the above homogenate binding studies. Analysis of autoradiograms from ECS treated animals show that alpha-1b adrenoceptors are increased in most cortical areas of the brain. Interestingly, alpha-1a adrenoceptors appear to be increased only in the amygdaloid nuclei. Chronic treatment with reserpine results in increases in {sup 3}H-prazosin binding in the cortex, thalamus, and ventral hippocampus whereas {sup 3}H-WB4101 binding is not altered in any of the brain regions surveyed.

• Research Organization: Georgetown Univ. Medical Center, Washington, DC (United States)
• OSTI ID: 5398581
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
AFFINITY
AMINES
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AUTORADIOGRAPHY
AZAARENES
AZOLES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BODY
BRAIN
CARDIOTONICS
CARDIOVASCULAR AGENTS
CENTRAL NERVOUS SYSTEM
DRUGS
ELECTRIC SHOCK
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
INDOLES
KINETICS
MAMMALS
MEMBRANE PROTEINS
NERVOUS SYSTEM
NEUROREGULATORS
NORADRENALINE
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
PYRROLES
RADIOPROTECTIVE SUBSTANCES
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SEROTONIN
SYMPATHOMIMETICS
TRITIUM COMPOUNDS
TRYPTAMINES
VERTEBRATES