EGF-receptor and extracellular matrix changes in mouse pulmonary carcinogenesis

Journal Article · Fri Mar 01 04:00:00 EST 1991 · Experimental Lung Research; (United States)

DOI:https://doi.org/10.3109/01902149109064422· OSTI ID:5395005

Smith, G J; Morris, C; Leigh, D; Rhodes, G C; Wong, A ^[1]

---

1. Carcinogenesis Research Unit, School of Pathology, University of New South Wales, Kensington (Australia)

Malignant Balb/c mouse lung cell clones related to alveologenic carcinoma exhibited low levels of epidermal growth factor (EGF) receptor activity compared to nonmalignant cell clones. Immunoprecipitation of cell homogenates and immunohistochemistry on urethane-induced lung tumors suggest that the absence of activity reflects decreased amounts of EGF receptor protein. Low levels of EGF receptor alone cannot cause neoplastic transformation, since a nonneoplastic cell cone, B5D3, exhibited low levels of EGF receptor despite its nontransformed phenotype. The reduced levels of EGF receptor in malignant clones have been mimicked by long-term (12 h) treatment of a nontransformed cell clone with 200 nM phorbol dibutyrate. The detection of mutated ras oncogene in the transformed cell lines, taken together with the EGF receptor findings, suggests that more than one alteration in the signal transduction pathway may be necessary for transformation in alveologenic adenoma and carcinoma cell systems. A further phenotypic feature of transformation, reduced expression of the extracellular matrix proteins fibronectin and laminin, may be mediated at the transcriptional level.

• OSTI ID: 5395005
• Journal Information: Experimental Lung Research; (United States), Journal Name: Experimental Lung Research; (United States) Vol. 17:2; ISSN 0190-2148; ISSN EXLRD
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
BIOLOGICAL PATHWAYS
BODY
CARBAMATES
CARBONIC ACID DERIVATIVES
CARBOXYLIC ACID SALTS
CARCINOGENESIS
CELL CULTURES
CELL TRANSFORMATIONS
CHEMICAL REACTIONS
CLONE CELLS
EPIDERMIS
EPITHELIUM
EXPERIMENTAL NEOPLASMS
GENES
GROWTH FACTORS
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LUNGS
MAMMALS
MEMBRANE PROTEINS
MICE
MITOGENS
ONCOGENES
ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATHOGENESIS
PHENOTYPE
PHOSPHORYLATION
PROTEINS
RECEPTORS
RESPIRATORY SYSTEM
RODENTS
SKIN
TISSUES
TRACER TECHNIQUES
URETHANE
VERTEBRATES