Impaired hepatic handling and processing of lysophosphatidylcholine in rats with liver cirrhosis

Journal Article · Mon Jul 01 00:00:00 EDT 1991 · Gastroenterology; (United States)

OSTI ID:5394897

Angelico, M; Alvaro, D; Cantafora, A; Masella, R; Gaudio, E; Gandin, C; Ginanni Corradini, S; Ariosto, F; Riggio, O; Capocaccia, L ^[1]

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1. II Division of Gastroenterology, University of Rome La Sapienza (Italy)

Lysophosphatidylcholine is a major metabolic product in the plasma and cellular turnover of phospholipids, with well-known membrane-toxic and proinflammatory properties. Because the liver plays a key role in plasma lysophosphatidylcholine removal and biotransformation and because virtually nothing is known of these processes in a diseased organ, the hepatobiliary metabolism of lysophosphatidylcholine was investigated in rats with carbon tetrachloride-induced liver cirrhosis. Twelve adult male Wistar rats with histologically confirmed cirrhosis and 8 control animals were fitted with jugular and biliary catheters and allowed to recover. The animals were kept under constant IV infusion of taurocholate (1 mumol/min). Two microcuries of sn-1{sup 14}Cpalmitoyl-lysophosphatidylcholine was administered as a single bolus. The fate of the injected radioactivity, including removal from plasma, uptake, and subcellular location in the liver and molecular and aggregative forms, was studied by combined chromatographic and radiochemical methods. Major findings were (a) that lysophosphatidylcholine has a prolonged permanence in plasma of cirrhotic rats, due both to decreased hepatic clearance and to depressed conversion into phosphatidylcholine; (b) that the rate of lysophosphatidylcholine acylation is much slower in the cirrhotic than in the normal liver, both at the microsomal and at the cytosolic level; (c) that cytosolic lysophosphatidylcholine in the cirrhotic liver, but not in the normal liver, is predominantly non-protein bound; (d) that the strict molecular selectivity of lysophosphatidylcholine acylation observed in controls is partially lost in cirrhosis; and (e) that a consistent fraction of lysophosphatidylcholine is converted into triacylglycerols in cirrhotics but not in controls.

• OSTI ID: 5394897
• Journal Information: Gastroenterology; (United States), Vol. 101:1; ISSN 0016-5085
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
CARBON TETRACHLORIDE
TOXICITY
LIVER CIRRHOSIS
PATHOGENESIS
PHOSPHOLIPIDS
METABOLISM
ACYLATION
CARBON 14 COMPOUNDS
RATS
TRACER TECHNIQUES
ANIMALS
CARBON COMPOUNDS
CHEMICAL REACTIONS
CHLORINATED ALIPHATIC HYDROCARBONS
DIGESTIVE SYSTEM DISEASES
DISEASES
ESTERS
HALOGENATED ALIPHATIC HYDROCARBONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
MAMMALS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
RODENTS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550901 - Pathology- Tracer Techniques