Further studies of the turnover of dog antithrombin III. Study of /sup 131/I-labelled antithrombin protease complexes

Journal Article · Fri Apr 15 04:00:00 EST 1983 · Thromb. Res.; (United States)

DOI:https://doi.org/10.1016/0049-3848(83)90239-6· OSTI ID:5394649

Leonard, B; Bies, R; Carlson, T; Reeve, E B

Fresh plasma containing /sup 131/I-antithrombin III (*I-AT) was coagulated and incubated at 37 degrees C for 2 hr. A ''complex peak,'' separated on heparin-agarose contained AT and *I-AT antigen but no heparin cofactor activity. Crossed immunoelectrophoresis showed only AT complexes. SDS PAGE showed 80% of the *I-AT in a major band (approximately 80,000 daltons), 15% in a minor band (approximately 100,000 daltons) and the rest in trace bands (approximately 60,000 and/or 115,000 daltons). Ammonia treatment of the complex peak released alpha-thrombin. After i.v. injection 80% of the complexed *I-AT, chiefly as the major band, left the plasma with t 1/2 approximately 15 min and was almost immediately catabolized to low molecular weight breakdown products. A major catabolic site was the liver. A simple kinetic model describes the findings approximately.

• Research Organization: Department of Medicine, University of Colorado Health Sciences Center, Denver
• OSTI ID: 5394649
• Journal Information: Thromb. Res.; (United States), Journal Name: Thromb. Res.; (United States) Vol. 30:2; ISSN THBRA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL HALF-LIFE
BODY
COAGULANTS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DOGS
DRUGS
ENZYMES
GLANDS
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROLASES
INTERMEDIATE MASS NUCLEI
IODINE 131
IODINE ISOTOPES
ISOTOPES
KINETICS
LABELLING
LIVER
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PEPTIDE HYDROLASES
RADIOISOTOPES
REACTION KINETICS
SERINE PROTEINASES
THROMBIN
VERTEBRATES