/sup 3/H-imipramine uptake into rat striatal slices and imipramine-induced /sup 3/H-dopamine efflux

Journal Article · Fri Apr 01 04:00:00 EST 1983 · Res. Commun. Chem. Pathol. Pharmacol.; (United States)

OSTI ID:5394648

Saito, R; Kawasaki, K; Ono, N; Kamiya, H

The effect of imipramine on spontaneous efflux of radiolabelled dopamine (DA) from slices of rat striatum was examined by a superfusion method. Imipramine at concentrations of 10 - 100 microM enhanced the efflux of DA accumulated in a high-affinity uptake system in a concentration-dependent manner. This efflux of /sup 3/H-DA was not affected by conditions (Ca/sup 2 +/-free medium, 100 microM bretylium and 30 microM tetrodotoxin) which inhibited the release of /sup 3/H-DA by electrical stimulation. Furthermore, this imipramine-induced /sup 3/H-DA efflux was temperature-dependent. The uptake of /sup 3/H-imipramine into striatal slices was determined. This uptake was concentration- and temperature-dependent and increased linearly. These results are discussed in relation to the hypothesis that /sup 3/H-DA efflux by imipramine is connected with uptake of imipramine.

• Research Organization: Department of Pharmacology, School of Pharmaceutical Sciences, Fukuoka University, Japan
• OSTI ID: 5394648
• Journal Information: Res. Commun. Chem. Pathol. Pharmacol.; (United States), Journal Name: Res. Commun. Chem. Pathol. Pharmacol.; (United States) Vol. 40:1; ISSN RCOCB
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMALS
ANTIDEPRESSANTS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOLOGICAL EFFECTS
BODY
BRAIN
CARDIOTONICS
CARDIOVASCULAR AGENTS
CENTRAL NERVOUS SYSTEM
CENTRAL NERVOUS SYSTEM AGENTS
CLEARANCE
DOPAMINE
DRUGS
EXCRETION
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IMIPRAMINE
LABELLED COMPOUNDS
LABELLING
MAMMALS
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHENOLS
POLYPHENOLS
PSYCHOTROPIC DRUGS
RATS
RODENTS
SYMPATHOMIMETICS
TEMPERATURE DEPENDENCE
TRITIUM COMPOUNDS
VERTEBRATES