Effects of neurotransmitters on calcium efflux from cultured glioma cells

Journal Article · Thu Jan 01 04:00:00 EST 1981 · J. Neurosci. Res.; (United States)

DOI:https://doi.org/10.1002/jnr.490060303· OSTI ID:5394232

Lazarewicz, J W; Kanje, M

The effects of various neurotransmitters and cyclic nucleotides on 45Ca2+ efflux in cultured human glioma cells were investigated. Glutamate and glycine, but not GABA, stimulated 45Ca2+ release from the cells. Stimulation of beta-adrenergic receptors but not alpha-adrenergic receptors also increased 45Ca2+ efflux. Cholinergic receptor stimulation by carbachol had the same effect. The stimulatory effect of carbachol was abolished in the presence of either atropine or hexamethonium. C-AMP and c-GMP increased the 45Ca2+ efflux, suggesting that these agents are involved in the transmitter-stimulated release of 45Ca2+ from the cell. Kinetic analysis of the efflux revealed four calcium compartments. The carbachol-stimulated efflux represented a net release of calcium and could be ascribed to the slowest compartment. The physiological role of the transmitter-stimulated calcium release is discussed in terms of calcium-regulated stimulus-response coupling in glial-neural interaction during excitation.

• Research Organization: Medical Research Centre, Polish Academy of Sciences, Warsaw
• OSTI ID: 5394232
• Journal Information: J. Neurosci. Res.; (United States), Journal Name: J. Neurosci. Res.; (United States) Vol. 6:3; ISSN JNRED
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH ISOTOPES
AMP
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CALCIUM 45
CALCIUM IONS
CALCIUM ISOTOPES
CELL CULTURES
CHARGED PARTICLES
COMPARTMENTS
DAYS LIVING RADIOISOTOPES
DISEASES
EVEN-ODD NUCLEI
GLIOMAS
INTERMEDIATE MASS NUCLEI
IONS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
NEOPLASMS
NUCLEI
NUCLEOTIDES
ORGANIC COMPOUNDS
PHYSIOLOGY
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
STIMULI
TRACER TECHNIQUES
TUMOR CELLS