Aneuploidy detection in human sperm nuclei using PRINS technique

Journal Article · Fri Aug 23 00:00:00 EDT 1996 · American Journal of Medical Genetics

DOI:https://doi.org/10.1002/(SICI)1096-8628(19960823)64:3<488::AID-AJMG8>3.3.CO;2-Z· OSTI ID:539406

Girardet, A; Coignet, L; Andreo, B ^[1]

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1. CNRS, Montpellier (France); and others

Rapid and specific identification of chromosomes can be attained in situ using the PRimed IN Situ (PRINS) labelling technique. We have adapted this technique to mature human sperm in combination with a protocol for simultaneous decondensation and denaturation of sperm nuclei. This strategy allowed us to obtain double labelling of human spermatozoa in a <2-hr reaction. In the present study, we report the estimates of disomy for chromosomes 3, 7, 10, 11, and 17 on 64,642 spermatozoa from 2 normal males. The incidences of disomy ranged from 0.28-0.34%. There were no significant interindividual or interchromosomal differences in disomy rates. 27 refs., 1 fig., 3 tabs.

• OSTI ID: 539406
• Journal Information: American Journal of Medical Genetics, Journal Name: American Journal of Medical Genetics Journal Issue: 3 Vol. 64; ISSN 0148-7299; ISSN AJMGDA
• Country of Publication: United States
• Language: English

Related Subjects

44 INSTRUMENTATION
INCLUDING NUCLEAR AND PARTICLE DETECTORS
55 BIOLOGY AND MEDICINE
BASIC STUDIES
ANEUPLOIDY
CELL NUCLEI
CHROMOSOMAL ABERRATIONS
DETECTION
DIPLOIDY
DNA SEQUENCERS
DNA SEQUENCING
EFFICIENCY
EVALUATION
FLUORESCENCE
HUMAN CHROMOSOMES
IN-SITU HYBRIDIZATION
KARYOTYPE
MEIOSIS
MUTATION FREQUENCY
NON-DISJUNCTION
OLIGONUCLEOTIDES
PROBES
SPERMATOZOA