Repair of ultraviolet light damage to the DNA of cultured human epidermal keratinocytes and fibroblasts

Journal Article · Mon Jan 01 04:00:00 EST 1979 · J. Invest. Dermatol.; (United States)

DOI:https://doi.org/10.1111/1523-1747.ep12514242· OSTI ID:5393869

Taichman, L B; Setlow, R B

Pure cultures of dermal fibroblasts and epidermal keroatinocytes have been obtained from a single biopsy of newborn foreskin. The cells were labeled, exposed to several doses of uv light, and allowed to repair in the dark for 16 h. The number of pyrimidine dimers before and after repair was assessed by measuring the numbers of sites in the DNA sensitive to a specific uv endonuclease. At all doses used, the extent of repair was similar in the cultured keratinocytes and cultured fibroblasts.

• Research Organization: State Univ. of New York, Stony Brook
• OSTI ID: 5393869
• Journal Information: J. Invest. Dermatol.; (United States), Journal Name: J. Invest. Dermatol.; (United States) Vol. 73:3; ISSN JIDEA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CELL CULTURES
CONNECTIVE TISSUE CELLS
DIMERS
DNA
DOSE-RESPONSE RELATIONSHIPS
DOSES
ELECTROMAGNETIC RADIATION
ENZYMES
EPIDERMIS
EPITHELIUM
FIBROBLASTS
HETEROCYCLIC COMPOUNDS
MAMMALS
MAN
NUCLEASES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHOSPHOTRANSFERASES
PRIMATES
PYRIMIDINES
RADIATION DOSES
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RECOVERY
REPAIR
SKIN
SOMATIC CELLS
TISSUES
TRANSFERASES
ULTRAVIOLET RADIATION
VERTEBRATES