Augmented stress fiber arrays after cytopharmacologic disassembly of microtubules

Conference · Sat Mar 01 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5393169

Godman, G C; Tannenbaum, J; Brett, J B

Disruption of microtubules (mt) of bovine aortic endothelial (BAE) cells, and normal and transformed fibroblasts, by exposure to 2.5 ..mu..M colchicine; 12 ..mu..M vinblastine; or 1 ..mu..M nocodazole, for 5 or 20 hrs results in aggregation of vimentin-intermediate filament (IF) and the development of markedly augmented stress fiber (SF) arrays. After disassembly of mt, confluent BAE, with circumferential marginal microfilament bands and few central SF, develop dense ribbon-like SF arrays, and spontaneously transformed fibroblasts (tHmf-e), which before treatment are apolar or epithelioid and have few or no SF, acquire extensive organized SF arrays. The axially oriented SF span the entire cell length and terminate in vinculin-containing adhesion plaques, polarizing these cells. The visible increase in SF associated actin is not accompanied by an increase either in actin synthesis (determined from electropherograms after pulse labeling with (/sup 35/S)methionine), or content (DNAse I assay for total cell actin). The reorganization of actin into SF and the development of vinculin adhesion plaques is independent of protein synthesis and occurs in the presence of cycloheximide (10 ..mu..g/ml). These results suggest a role for mt and IF in the regulation of the organizational state of the actin-based cytoskeleton.

• Research Organization: Columbia Univ., New York, NY
• OSTI ID: 5393169
• Report Number(s): CONF-8604222-; TRN: 86-028376
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ACTIN
BIOSYNTHESIS
ANTINEOPLASTIC DRUGS
BIOLOGICAL EFFECTS
COLCHICINE
MICROTUBULES
BIOLOGICAL STRESS
ADHESION
AORTA
CATTLE
ENDOTHELIUM
FIBROBLASTS
METHIONINE
SULFUR 35
TRACER TECHNIQUES
ALKALOIDS
AMINO ACIDS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIMITOTIC DRUGS
ANTIPYRETICS
ARTERIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD VESSELS
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
DRUGS
EVEN-ODD NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMALS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
RUMINANTS
SOMATIC CELLS
SULFUR ISOTOPES
SYNTHESIS
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques