Comparison of the canine and human acid {beta}-galactosidase gene

Journal Article · Fri May 17 00:00:00 EDT 1996 · American Journal of Medical Genetics

DOI:https://doi.org/10.1002/(SICI)1096-8628(19960517)63:2<340::AID-AJMG3>3.3.CO;2-Y· OSTI ID:539187

Ahern-Rindell, A J ^[1]; Kretz, K A ^[2]; O`Brien, J S ^[3]

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1. Weber State Univ., Ogden, UT (United States)
2. Recombinant BioCatalysis, Inc., La Jolla, CA (United States)
3. Univ. of California at San Diego, La Jolla, CA (United States)

Several canine cDNA libraries were screened with human {beta}-galactosidase cDNA as probe. Seven positive clones were isolated and sequenced yielding a partial (2060 bp) canine {beta}-galactosidase cDNA with 86% identity to the human {beta}-galactosidase cDNA. Preliminary analysis of a canine genomic library indicated conservation of exon number and size. Analysis by Northern blotting disclosed a single mRNA of 2.4 kb in fibroblasts and liver from normal dogs and dogs affected with GM1 gangliosidosis. Although incomplete, these results indicate canine GM1 gangliosidosis is a suitable animal model of the human disease and should further efforts to devise a gene therapy strategy for its treatment. 20 refs., 2 figs., 1 tab.

• OSTI ID: 539187
• Journal Information: American Journal of Medical Genetics, Vol. 63, Issue 2; Other Information: PBD: 17 May 1996
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
DOGS
CORRELATIONS
GALACTOSIDASE
DNA-CLONING
DNA SEQUENCING
STRUCTURE-ACTIVITY RELATIONSHIPS
GENETIC MAPPING
HUMAN POPULATIONS
METABOLIC DISEASES
HEREDITARY DISEASES
LYSOSOMES
THERAPY
METABOLISM
BIOLOGICAL MODELS
GENETICS
PROBES
EXONS
GENETIC ENGINEERING
MESSENGER-RNA
RECESSIVE MUTATIONS
DNA HYBRIDIZATION
NUCLEOTIDES