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Isolation of a bacterium that reductively dechlorinates tetrachloroethene to ethene

Journal Article · · Science
; ;  [1]
  1. Cornell Univ., Ithaca, NY (United States); and others

Tetrachloroethene is a prominent groundwater pollutant that can be reductively dechlorinated by mixed anaerobic microbial populations to the nontoxic product ethene. Strain 195, a coccoid bacterium that dechlorinates tetrachlorethene to ethene, was isolated and characterized. Growth of strain 195 with H{sub 2} and tetrachloroethene as the electron donor and acceptor pair required extracts from mixed microbial cultures. Growth of strain 195 was resistant to ampicillin and vancomycin; its cell wall did not react with a peptidoglycan-specific lectin and its ultrastructure resembled S-layers of Archaea. Analysis of the 16S ribosomal DNA sequence of strain 195 indicated that it is a eubacterium without close affiliation to any known groups. 24 refs., 4 figs., 1 tab.

OSTI ID:
539119
Journal Information:
Science, Journal Name: Science Journal Issue: 5318 Vol. 276; ISSN SCIEAS; ISSN 0036-8075
Country of Publication:
United States
Language:
English

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