18S rRNA degradation is not accompanied by altered rRNA transport at early times following irradiation of HeLa cells

Technical Report · Mon Jan 01 04:00:00 EST 1990

OSTI ID:5390469

Fuchs, P; Krolak, J M; McClain, D; Minton, K W

In recent investigations on the effects of radiation on rRNA processing in HeLa S3 cells, the authors pulse-labeled the cells with uridine immediately prior to irradiation. The 45 S rRNA precursor, which undergoes nuclear processing to form one each of its major daughter species, 28S and 18S rRNA, was separated from the daughter species by gel electrophoresis and the radiolabel in each species determined at various times after irradiation. By pulse-labeling the cells prior to irradiation, superimposed effects caused by radiation-induced alterations of rRNA transcription and Refs. therein were minimized, permitting selective analysis of the processing of that fraction of 45S precursor that had been synthesized (radiolabeled) predominantly prior to irradiation. They now report more detailed studies on 45S rRNA processing within the first 2 h following irradiation in which they have found a maximum 28 S:18 S ratio of 2:1 that is observed about 1 h following irradiation of 5 or 10 Gy.

• Research Organization: Armed Forces Radiobiology Research Inst., Bethesda, MD (United States)
• OSTI ID: 5390469
• Report Number(s): AD-A-235466/0/XAB; AFRRI-SR--91-6
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
ELECTROPHORESIS
HELA CELLS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IRRADIATION
MEMBRANE TRANSPORT
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRECURSOR
PYRIMIDINES
RADIATION EFFECTS
RADIOSENSITIVITY EFFECTS
RIBOSIDES
RIBOSOMAL RNA
RNA
TRANSCRIPTION
URACILS
URIDINE