Influence of administered indigenous microorganisms on uptake of (iodine-125). gamma. -globulin in vivo by intestinal segments of neonatal calves

Journal Article · Thu Jan 01 04:00:00 EST 1981 · J. Dairy Sci.; (United States)

DOI:https://doi.org/10.3168/jds.S0022-0302(81)82528-3· OSTI ID:5389925

James, R E; Polan, C E; Cummins, K A

Ten calves less than 14 h of age (average 8.6 h) were anaesthetized, and the intestine was ligated into segments 10 cm in length at 3-cm intervals beginning 1.8 m anterior to the ileocecal junction and proceeding proximally. Seven treatments were assigned in random order to segments in three successive sections of the small intestine. Segments received 1 ml of viable bacteria of intestinal origin, autoclaved bacteria of intestinal origin, or sterile microbiological broth at zero time; then after 4 h they were injected with iodine-125 labeled ..gamma..-globulin. After an additional 1.5 h, the experiment was ended and uptake assayed. Two treatments measured anaerobic microbial growth after 4 h incubation with 1 ml of either sterile broth or live bacteria culture. Residual (iodine-125) ..gamma..-globulin was measured in segments receiving 1 ml of sterile broth or live bacteria culture with 5.5-h incubation followed by 15-s exposure to labeled ..gamma..-globulin. Uptake was lowest in segments receiving live bacteria as compared to segments receiving sterile inocula. Number of bacteria per gram of tissue was correlated negatively with uptake. Low corticosteroids in serum were associated with low uptake of ..gamma..-globulin.

• Research Organization: Virginia Polytechnic Inst., Blacksburg
• OSTI ID: 5389925
• Journal Information: J. Dairy Sci.; (United States), Journal Name: J. Dairy Sci.; (United States) Vol. 64:1; ISSN JDSCA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BACTERIA
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CATTLE
CHEMICAL REACTIONS
CHEMISORPTION
COWS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
GASTROINTESTINAL TRACT
GLOBULINS
GLOBULINS-GAMMA
IMMUNITY
INFECTIVITY
INOCULATION
INTERMEDIATE MASS NUCLEI
INTESTINES
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
MAMMALS
MICROORGANISMS
NEONATES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
RUMINANTS
SEPARATION PROCESSES
SMALL INTESTINE
SORPTION
TRACER TECHNIQUES
VERTEBRATES