Rapid column chromatographic method for the isolation of catechol-type siderophores

Journal Article · Fri Jun 01 00:00:00 EDT 1979 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(79)90740-1· OSTI ID:5389874

Robinson, A V

A column chromatographic method using a polyamide support has been developed which allows rapid isolation of catechol-type siderophores directly from culture supernatants. The method is superior to presently used methods with regard to speed, convenience, and lack of degradation products. Siderophores from Micrococcus denitrificans (ATCC 19367), Klebsiella aerogenes (ATCC 13882), and Bacillus subtilis (ATCC 6051) have been purified and the method has been tested with two synthetic catechol-type chelators, N/sup 1/,N/sup 2/-bis(2,3-dihydroxybenzoyl)ethylenediamine and N/sup 1/,N/sup 4/-bis(2,3-dihydroxybenzoyl)putrescine.

• Research Organization: Battelle Pacific Northwest Lab., Richland, WA
• DOE Contract Number: EY-76-C-06-1830
• OSTI ID: 5389874
• Journal Information: Anal. Biochem.; (United States), Vol. 95:2
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
59 BASIC BIOLOGICAL SCIENCES
EXTRACTION COLUMNS
EFFICIENCY
PYROCATECHOL
CHEMICAL PREPARATION
BACILLUS SUBTILIS
CELL CULTURES
CHELATING AGENTS
CHEMICAL REACTION KINETICS
COLUMN PACKING
DECONTAMINATION
EXPERIMENTAL DATA
ISOLATED VALUES
MICROCOCCUS
POLYAMIDES
TIME DEPENDENCE
AROMATICS
BACILLUS
BACTERIA
CLEANING
DATA
DATA FORMS
DEVELOPERS
EXTRACTION APPARATUSES
HYDROXY COMPOUNDS
INFORMATION
KINETICS
MICROORGANISMS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC POLYMERS
PACKINGS
PHENOLS
POLYMERS
POLYPHENOLS
REACTION KINETICS
SYNTHESIS
550600* - Medicine
550200 - Biochemistry