Rapid column chromatographic method for the isolation of catechol-type siderophores
Journal Article
·
· Anal. Biochem.; (United States)
A column chromatographic method using a polyamide support has been developed which allows rapid isolation of catechol-type siderophores directly from culture supernatants. The method is superior to presently used methods with regard to speed, convenience, and lack of degradation products. Siderophores from Micrococcus denitrificans (ATCC 19367), Klebsiella aerogenes (ATCC 13882), and Bacillus subtilis (ATCC 6051) have been purified and the method has been tested with two synthetic catechol-type chelators, N/sup 1/,N/sup 2/-bis(2,3-dihydroxybenzoyl)ethylenediamine and N/sup 1/,N/sup 4/-bis(2,3-dihydroxybenzoyl)putrescine.
- Research Organization:
- Battelle Pacific Northwest Lab., Richland, WA
- DOE Contract Number:
- EY-76-C-06-1830
- OSTI ID:
- 5389874
- Journal Information:
- Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 95:2; ISSN ANBCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200 -- Biochemistry
550600* -- Medicine
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
AROMATICS
BACILLUS
BACILLUS SUBTILIS
BACTERIA
CELL CULTURES
CHELATING AGENTS
CHEMICAL PREPARATION
CHEMICAL REACTION KINETICS
CLEANING
COLUMN PACKING
DATA
DATA FORMS
DECONTAMINATION
DEVELOPERS
EFFICIENCY
EXPERIMENTAL DATA
EXTRACTION APPARATUSES
EXTRACTION COLUMNS
HYDROXY COMPOUNDS
INFORMATION
ISOLATED VALUES
KINETICS
MICROCOCCUS
MICROORGANISMS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC POLYMERS
PACKINGS
PHENOLS
POLYAMIDES
POLYMERS
POLYPHENOLS
PYROCATECHOL
REACTION KINETICS
SYNTHESIS
TIME DEPENDENCE
550600* -- Medicine
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
AROMATICS
BACILLUS
BACILLUS SUBTILIS
BACTERIA
CELL CULTURES
CHELATING AGENTS
CHEMICAL PREPARATION
CHEMICAL REACTION KINETICS
CLEANING
COLUMN PACKING
DATA
DATA FORMS
DECONTAMINATION
DEVELOPERS
EFFICIENCY
EXPERIMENTAL DATA
EXTRACTION APPARATUSES
EXTRACTION COLUMNS
HYDROXY COMPOUNDS
INFORMATION
ISOLATED VALUES
KINETICS
MICROCOCCUS
MICROORGANISMS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC POLYMERS
PACKINGS
PHENOLS
POLYAMIDES
POLYMERS
POLYPHENOLS
PYROCATECHOL
REACTION KINETICS
SYNTHESIS
TIME DEPENDENCE