Absence of carbonic anhydrase on the surface of red cells suspended in an elutriator

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5389522

Dodek, P; Effros, R M

Studies of the carbonic anhydrase activity of intact red cells are complicated by hemolysis, which markedly accelerates catalytic conversion between HCO/sub 2//sup -/ and CO/sub 2/. The authors have reduced this effect to negligible levels by studying red cells suspended with an elutriator. 1.Oml of packed rabbit red cells were introduced in an elutriator chamber which was spun in a centrifuge with a force of 2900G. An isotonic, pH 7.4, 6/sup 0/C, HCO/sub 3//sup -/ Ringer's lactate solution was pumped centripetally through the chamber to keep the cells in suspension. 0.1 ml of the perfusion solution containing /sup 22/Na/sup +/, /sup 36/Cl-, H/sup 14/CO/sub 3//sup -///sup 14/CO/sub 2/ (equilibrated), and /sup 3/H/sub 2/O were injected into the elutriator inflow and 22 samples were collected with a computerized anaerobic collector. Outflow concentrations of each radionuclide were divided by the amounts injected to yield fractional concentrations and upslope areas of indicators were divided by that of /sup 22/Na/sup +/ to yield an index of red cell exchange, R. Under control conditions, R(/sup 14/C) averaged .80 +/- .01 (S.E.). After anion exchange had been blocked with 0.1..mu..M DIDS, R(/sup 14/C) increased to .86 +/- .01. When 100 mg/1 bovine carbonic anhydrase was added R(/sup 1/$C) fell to .78 +/- .01. Subsequent addition of acetazolamide (10/sup -5/) increased R(/sup 14/C) to .86 +/- .01. R(/sup 36/Cl/sup -/) was increased from .91 +/- .02 to .96 +/- .01 after DIDS. These data indicate no detectable carbonic anhydrase activity on red cell surface.

• Research Organization: Univ. of California Medical Center, Torrance
• OSTI ID: 5389522
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL ISOTOPES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBON-OXYGEN LYASES
CARBONIC ANHYDRASE
CATTLE
CHALCOGENIDES
CHLORINE 36
CHLORINE ISOTOPES
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
ELUTRIATION
ENZYME ACTIVITY
ENZYMES
ERYTHROCYTES
HEMOLYSIS
HYDRO-LYASES
ION EXCHANGE
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LYASES
LYSIS
MAMMALS
MATERIALS
NUCLEI
ODD-ODD NUCLEI
OXIDES
OXYGEN COMPOUNDS
PATHOLOGICAL CHANGES
RABBITS
RADIOISOTOPES
RUMINANTS
SEPARATION PROCESSES
SODIUM 22
SODIUM ISOTOPES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
YEARS LIVING RADIOISOTOPES