Cloning of a gene cluster encoding biphenyl and chlorobiphenyl degradation in Pseudomonas pseudoalcaligenes
Journal Article
·
· J. Bacteriol.; (United States)
OSTI ID:5387914
A gene cluster encoding biphenyl- and chlorobiphenyl-degrading enzymes was cloned from a soil pseudomonad into pseudomonas aeruginosa PA01161. Chromosomal DNA from polychlorinated biphenyl-degrading Pseudomonas pseudoalcaligenes KF707 was digested with restriction endonuclease XhoI and cloned into the unique XhoI site of broad-host-range plasmid pKF330. Of 8000 transformants tested, only 1, containing the chimeric plasmid pMFB1, rendered the host cell able to convert biphenyls and chlorobiphenyls to ring meta cleavage compounds via dihydrodiols and dihydroxy compounds. The chimeric plasmid contained a 7.9-kilobase XhoI insert. Subcloning experiments revealed that the genes bphA (encoding biphenyl dioxygenase), bphB (encoding dihydrodiol dehydrogenase), and bphC (encoding 2,3-dihydroxybiphenyl dioxygenase) were coded for by the 7.9-kilobase fragment. The gene order was bphA-bphB-bphC. The hydrolase activity, which converted the intermediate meta cleavage compounds to the final product, chlorobenzoic acids, and was encoded by a putative bphD gene, was missing from the cloned 7.9-kilobase fragment.
- Research Organization:
- Fermentation Research Institute, Tsukuba, Japan
- OSTI ID:
- 5387914
- Journal Information:
- J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 166:2; ISSN JOBAA
- Country of Publication:
- United States
- Language:
- English
Similar Records
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Purification and properties of 2,3-dihydroxybiphenyl dioxygenase from polychlorinated biphenyl-degrading Pseudomonas pseudoalcaligenes and Pseudomonas aeruginosa carrying the cloned bphC gene
Identification and localization of 3-phenylcatechol dioxygenase and 2-hydroxy-6-oxo-6-phenylhexa-2, 4-dienoate hydrolase genes of pseudomonas putida and expression in escherichia coli
Journal Article
·
Wed Jan 31 23:00:00 EST 1990
· Journal of Bacteriology; (USA)
·
OSTI ID:7016632
Purification and properties of 2,3-dihydroxybiphenyl dioxygenase from polychlorinated biphenyl-degrading Pseudomonas pseudoalcaligenes and Pseudomonas aeruginosa carrying the cloned bphC gene
Journal Article
·
Sat Jan 31 23:00:00 EST 1987
· J. Bacteriol.; (United States)
·
OSTI ID:6470076
Identification and localization of 3-phenylcatechol dioxygenase and 2-hydroxy-6-oxo-6-phenylhexa-2, 4-dienoate hydrolase genes of pseudomonas putida and expression in escherichia coli
Journal Article
·
Sat Mar 31 23:00:00 EST 1990
· Applied and Environmental Microbiology; (USA)
·
OSTI ID:6605189
Related Subjects
550400 -- Genetics
560302* -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
BACTERIA
BIODEGRADATION
BIPHENYL
CATABOLISM
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHLORINATED AROMATIC HYDROCARBONS
CLONING
DECOMPOSITION
DNA-ASE
ENDONUCLEASES
ENZYMES
ESTERASES
GENES
HALOGENATED AROMATIC HYDROCARBONS
HYDROCARBONS
HYDROLASES
METABOLISM
MICROORGANISMS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
PHOSPHODIESTERASES
PLASMIDS
PSEUDOMONAS
560302* -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
BACTERIA
BIODEGRADATION
BIPHENYL
CATABOLISM
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHLORINATED AROMATIC HYDROCARBONS
CLONING
DECOMPOSITION
DNA-ASE
ENDONUCLEASES
ENZYMES
ESTERASES
GENES
HALOGENATED AROMATIC HYDROCARBONS
HYDROCARBONS
HYDROLASES
METABOLISM
MICROORGANISMS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
PHOSPHODIESTERASES
PLASMIDS
PSEUDOMONAS