Physical and chemical control of released microorganisms at field sites
An important consideration in the environmental release of a genetically engineered microorganism (GEM) is the capability for reduction or elimination of GEM populations once their function is completed or if adverse environmental effects are observed. The decontamination treatments of burning and biocide application, alone and in combination with tilling, were evaluated for their ability to reduce populations of bacteria released on the phylloplane. Field plots of bush beans sprayed with the bacterium Erwinia herbicola, received the following treatments: (1) control, (2) control + till, (3) burn, (4) burn + till, (5) Kocide (cupric hydroxide), (6) Kocide + till, (7) Agri-strep (streptomycin sulfate), and (8) Agri-strept + till. Leaves and soil from the plots were sampled -1, 1, 5, 8, 12, 15, 19, and 27 days after application of the decontamination treatments. Burning produced a significant and persistent reduction in the number of bacteria whereas tilling, alone or in combination with the biocide treatments, stimulated a significant and persistent reduction in the number of bacteria, whereas tilling, alone or in combination with the biocide treatments, stimulated a significant increase in bacterial populations that persisted for several weeks.
- Research Organization:
- Environmental Protection Agency, Corvallis, OR (United States). Environmental Research Lab.
- OSTI ID:
- 5384848
- Report Number(s):
- PB-92-153360/XAB; EPA-600/J-92/080
- Resource Relation:
- Other Information: Pub. in Canadian Jnl. of Microbiology, v37 p708-712. See also PB89-138465 and PB91-109975. Prepared in cooperation with NSI Technology Services Corp., Corvallis, OR. Environmental Research Lab
- Country of Publication:
- United States
- Language:
- English
Similar Records
Influence of Carbon and Microbial Community Priming on the Attenuation of Uranium in a Contaminated Floodplain Aquifer
The effects of precommercial thinning and midstory-control on the flora and fauna of young longleaf pine plantations.
Related Subjects
BACTERIA
GENETIC ENGINEERING
COMBUSTION
DECONTAMINATION
FIELD TESTS
LEGUMINOSAE
REGRESSION ANALYSIS
SOILS
BIOTECHNOLOGY
CHEMICAL REACTIONS
CLEANING
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MATHEMATICS
MICROORGANISMS
OXIDATION
PLANTS
STATISTICS
TESTING
THERMOCHEMICAL PROCESSES
550200* - Biochemistry