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Epidermal growth factor, bradykinin, and histamine stimulate inositol phosphate formation in A431 human epidermoid carcinoma cells

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5383554
; ; ;

Incubation of A431 cells with epidermal growth factor (EGF), bradykinin (BK), or histamine (HA) resulted in a 3-, 6-, or 3-fold increase, respectively, in the formation of the inositol phosphates (InsP), InsP/sub 1/, and InsP/sub 2/, and InsP/sub 3/. The K/sub 0.5/ values for stimulation were 3 nM, 3 nM, and 10 ..mu..M for EGF, BK, and HA, respectively. Total InsP formation was essentially linear for each hormone for at least 10 min; the accumulation of InsP/sub 3/ was maximal within 15 sec. The efflux of /sup 45/Ca/sup + +/ from /sup 45/Ca-preincubated cells was increased by all three hormones, suggesting that EGF-, BK-, and HA-stimulated breakdown of phosphoinositides results in Ca/sup + +/ mobilization in these cells. A431 cells should prove a useful model system for comparison of the mechanism(s) whereby EGF and other hormone receptors regulate phosphoinositide metabolism.

Research Organization:
Univ. of North Carolina, Chapel Hill
OSTI ID:
5383554
Report Number(s):
CONF-8604222-
Journal Information:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:3; ISSN FEPRA
Country of Publication:
United States
Language:
English

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Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
AMINES
ANIMAL CELLS
ANIMALS
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MODELS
BRADYKININ
CALCIUM 45
CALCIUM COMPOUNDS
CALCIUM ISOTOPES
CARBOHYDRATES
CARCINOMAS
CATIONS
CHARGED PARTICLES
DAYS LIVING RADIOISOTOPES
DISEASES
EVEN-ODD NUCLEI
HETEROCYCLIC COMPOUNDS
HISTAMINE
HORMONES
IMIDAZOLES
INOSITOLS
INTERMEDIATE MASS NUCLEI
IONS
ISOTOPE APPLICATIONS
ISOTOPES
KININS
MAMMALS
MAN
MEMBRANE TRANSPORT
METABOLISM
MONOSACCHARIDES
NEOPLASMS
NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDES
POLYPEPTIDES
PRIMATES
PROTEINS
RADIOISOTOPES
SACCHARIDES
TRACER TECHNIQUES
TUMOR CELLS
VERTEBRATES