Enzyme system generation of singlet (/sup 1/. delta. /sub g/) molecular oxygen observed directly by 1. 0-1. 8-. mu. m luminescence spectroscopy
The observation of a strong singlet molecular oxygen luminescence emission in the ir produced in the decomposition of hydrogen peroxide by the enzymes lactoperoxidase, catalase, and chloroperoxidase is reported. A mixture of H/sub 2/O and D/sub 2/O was used as a reaction media. The luminescence emission spectra of the lactoperoxidase/H/sub 2/O/sub 2/ and chloroperoxidase/H/sub 2/O/sub 2/ were found to exhibit a single emission band at 1.28 ..mu..m for the former and three bands for the latter - a strong band at 1.30 ..mu..m and a possible weak band extending from the long-wavelength edge of the monochromator at 1.60 to 1.45 ..mu..m. The peak at 1.28 ..mu..m is absent in the catalase/H/sub 2/O/sub 2/ spectrum, but the peak at 1.64 ..mu..m is very evident. The spectra are interpreted as indicating the generation of free singlet oxygen (peak at 1.28 and 1.30 ..mu..m) in the case of the first two enzymes/H/sub 2/O/sub 2/ systems and the generation of predominately bound singlet molecular oxygen in the case of catalase/H/sub 2/O/sub 2/.
- Research Organization:
- Florida State Univ., Tallahassee
- DOE Contract Number:
- AS05-78EV05855
- OSTI ID:
- 5383483
- Journal Information:
- J. Am. Chem. Soc.; (United States), Journal Name: J. Am. Chem. Soc.; (United States) Vol. 105:24; ISSN JACSA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
400201 -- Chemical & Physicochemical Properties
400202* -- Isotope Effects
Isotope Exchange
& Isotope Separation
CARBOXYLIC ACIDS
CATALASE
CHEMICAL REACTIONS
DECOMPOSITION
DEUTERIUM
ELEMENTS
ENZYMES
HEAVY WATER
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROGEN ISOTOPES
HYDROGEN PEROXIDE
INFRARED SPECTRA
ISOTOPE EFFECTS
ISOTOPES
LIGHT NUCLEI
LUMINESCENCE
NONMETALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
OXYGEN
OXYGEN COMPOUNDS
PEROXIDASES
PEROXIDES
PORPHYRINS
SPECTRA
STABLE ISOTOPES
WATER