Energy transduction by anaerobic ferric iron respiration in Thiobacillus ferrooxidans
- Delft Univ. of Technology (Netherlands)
Formate-grown cells of the obligately chemolithoautotrophic acidophile Thiobacillus ferrooxidans were capable of formate- and elemental sulfur-dependent reduction of ferric iron under anaerovic conditions. Under aerobic conditions, both oxygen and ferric iron could be simultaneously used as electron acceptors. To investigate whether anaerobic ferric iron respiration by T. ferrooxidans is an energy-transducing process, uptake of amino acids was studied. Glycine uptake by starved cells did not occur in the absence of an electron donor, neither under aerobic conditions nor under anaerobic conditions. Uptake of glycine could be driven by formate- and ferrous iron-dependent oxygen uptake. Under anaerobic conditions, ferric iron respiration with the electron donors formate and elemental sulfur could energize glycine uptake. Glycine uptake was inhibited by the uncoupler 2,4-dinitrophenol. The results indicate that anaerobic ferric iron respiration can contribute to the energy budget of T. ferrooxidans.
- OSTI ID:
- 5383203
- Journal Information:
- Applied and Environmental Microbiology; (United States), Journal Name: Applied and Environmental Microbiology; (United States) Vol. 57:7; ISSN 0099-2240; ISSN AEMID
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
540210* -- Environment
Terrestrial-- Basic Studies-- (1990-)
AMINO ACIDS
ANAEROBIC CONDITIONS
AROMATICS
BACILLUS
BACTERIA
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
ELEMENTS
FORMIC ACID
GLYCINE
HYDROXY COMPOUNDS
INHIBITION
IRON
MEMBRANE TRANSPORT
METABOLISM
METALS
MICROORGANISMS
MONOCARBOXYLIC ACIDS
NITRO COMPOUNDS
NITROPHENOL
NONMETALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHENOLS
REDUCTION
RESPIRATION
SULFUR
SULFUR-OXIDIZING BACTERIA
THIOBACILLUS FERROXIDANS
TRANSITION ELEMENTS
UPTAKE