Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Classifying mutagens as to their specificity in causing the six possible transitions and transversions: a simple analysis using the Salmonella mutagenicity assay

Journal Article · · Environ. Mutagen.; (United States)
;
The standard Salmonella tester strains used to detect base substitution mutations carry the hisG428 ochre mutation (TA102 and TA104) and the hisG46 missense mutation (TA100). These mutations can be reverted by base changes at their mutant his loci or at extragenic suppressor loci. The base changes resulting in each class of revertants of these mutations have been identified, and simple phenotypic screens have been developed to distinguish among them. These screens are the basis for a rapid and simple system for determining the base substitution specificity of mutagens using hisG428- and hisG46-containing tester strains. Diagnostic mutagens specific for each of the six possible base changes (transitions and transversions) have been identified. Using these diagnostic mutagens, two additional strains, each specifically reverted by a single base substitution mutation, have been developed to provide a minimum of two loci at which to detect each type of base change.The ability of this system to provide detailed information about mutational specificity in a variety of DNA repair backgrounds will allow further elucidation of the mechanisms of mutagenesis and DNA repair.
Research Organization:
Univ. of California, Berkeley
DOE Contract Number:
AS03-76EV70156
OSTI ID:
5382347
Journal Information:
Environ. Mutagen.; (United States), Journal Name: Environ. Mutagen.; (United States) Vol. 8:1; ISSN ENMUD
Country of Publication:
United States
Language:
English

Similar Records

Spontaneous and mutagen-induced deletions: mechanistic studies in Salmonella tester strain TA102
Journal Article · Sun Jul 01 00:00:00 EDT 1984 · Proc. Natl. Acad. Sci. U.S.A.; (United States) · OSTI ID:5997388
Toxicology Studies on Lewisite and Sulfur Mustard Agents: Mutagenicity of Sulfur Mustard in the Salmonella Histidine Reversion Assay (Final Report)
Technical Report · Mon Jul 31 00:00:00 EDT 1989 · OSTI ID:1086509
Heterocyclic amine mutagenicity/carcinogenicity: Influence of repair, metabolism, and structure
Conference · Thu Jan 14 23:00:00 EST 1993 · OSTI ID:10172842

Related Subjects

560302* -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
AROMATICS
BACTERIA
BENZOPYRENE
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CLASSIFICATION
CONDENSED AROMATICS
DNA REPAIR
DRUGS
EMS
ESTERS
HYDROCARBONS
MICROORGANISMS
MITOMYCIN
MUTAGEN SCREENING
MUTAGENESIS
MUTAGENS
MUTANTS
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RECOVERY
REPAIR
REVERTANTS
SALMONELLA
SALMONELLA TYPHIMURIUM
SCREENING
SULFONIC ACID ESTERS