Binding of cholesterol and inhibitory peptide derivatives with the fusogenic hydrophobic sequence of F-glycoprotein of HVJ (Sendai virus): possible implication in the fusion reaction
Specificity of the binding of sterols and related compounds with purified F-protein (fusion protein) of the HVJ (Sendai virus) was studied by binding competition with (/sup 3/H) cholesterol. Requirement for cholesterol or the A/B ring trans structure and nonrequirement for the 3-hydroxyl group were found in this binding. Binding of /sup 125/I-labeled Z-Phe-Tyr, an inhibitory peptide of viral membrane-cell membrane fusion, was studied by using purified proteins and virions. F-Protein and virions showed a specific binding with the peptide, whereas the result was negative with hemagglutinin and neuraminidase protein. Thermolysin-truncated F-protein (an F-protein derivative deprived of a 2.5-kDa fragment from the N-terminal of the F/sub 1/ subunit and without fusogenic activity) exhibited a considerably diminished binding ability both to cholesterol and to inhibitory peptides. Therefore, the N-terminal hydrophobic sequence that was previously assigned as fusogenic seems to be the binding site of these molecules. In support of this, the binding of cholesterol with F-protein was inhibited by Z-Phe-Tyr and other fusion inhibitory peptides, whereas it was not affected with non-fusion-inhibitory Z-Gly-Phe. These results are discussed in relation to the notion that the binding of the N-terminal portion of the F/sub 1/ subunit of F-protein with cholesterol in the target cell membranes facilitiates the fusion reaction.
- Research Organization:
- Kyoto Univ. (Japan)
- OSTI ID:
- 5381859
- Journal Information:
- Biochemistry; (United States), Vol. 27:4
- Country of Publication:
- United States
- Language:
- English
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CHOLESTEROL
CONFIGURATION INTERACTION
LABELLED COMPOUNDS
PROTEINS
CELL MEMBRANES
ELECTROPHORESIS
ENZYMATIC HYDROLYSIS
IODINE 125
SODIUM IODIDES
STEROLS
TRITIUM COMPOUNDS
VIRUSES
ALKALI METAL COMPOUNDS
BETA DECAY RADIOISOTOPES
CELL CONSTITUENTS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
ELECTRON CAPTURE RADIOISOTOPES
HALIDES
HALOGEN COMPOUNDS
HYDROLYSIS
HYDROXY COMPOUNDS
INORGANIC PHOSPHORS
INTERMEDIATE MASS NUCLEI
IODIDES
IODINE COMPOUNDS
IODINE ISOTOPES
ISOTOPES
LYSIS
MEMBRANES
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHORS
RADIOISOTOPES
SODIUM COMPOUNDS
SOLVOLYSIS
STEROIDS
550201* - Biochemistry- Tracer Techniques