Identification of an alpha sub 2 -adrenoceptor in human coronary arteries by radioligand binding assay

Journal Article · Tue Jan 01 04:00:00 EST 1991 · Life Sciences; (United States)

DOI:https://doi.org/10.1016/0024-3205(91)90606-C· OSTI ID:5381842

Ishikawa, Y; Umemura, S; Uchino, K; Shindou, T; Yasuda, G; Minamisawa, K; Hayashi, S; Hirawa, N; Ishii, M ^[1]

---

1. Yokohama City Univ. (JAPAN)

A single high affinity binding site for an alpha{sub 2}-adrenoceptor in human coronary arteries was identified by radioligand binding assay. Human coronary arteries were obtained at autopsy within 6 hours of death. A crude membrane solution was incubated with ({sup 3}H)-rauwolscine at 25C for 30 min. The binding of ({sup 3}H)-rauwolscine was rapidly saturable and reversible. Kd was 1.2 {plus minus} 0.2 (SE) nM and Bmax 22 {plus minus} 3 fmol/mg protein. This is the first study which has shown the presence of an alpha{sub 2}-adrenoceptor in human coronary arteries using a radioligand binding assay method.

• OSTI ID: 5381842
• Journal Information: Life Sciences; (United States), Journal Name: Life Sciences; (United States) Vol. 48:26; ISSN 0024-3205; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ANIMALS
ARTERIES
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMICAL REACTION KINETICS
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
CELL MEMBRANES
CORONARIES
DRUGS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
MAN
MEMBRANE PROTEINS
MEMBRANES
ORGANIC COMPOUNDS
ORGANS
PRIMATES
PROTEINS
RADIOASSAY
REACTION KINETICS
RECEPTORS
SYMPATHOMIMETICS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES