Molecular cloning of the human gene for von Willebrand factor and identification of the transcription initiation site

Journal Article · Wed Jul 01 00:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.13.4393· OSTI ID:5381470

Collins, C J; Underdahl, J P; Levene, R B; Ravera, C P; Morin, M J; Dombalagian, M J; Ricca, G; Livingston, D M; Lynch, D C

A series of overlapping cosmid genomic clones have been isolated that contain the entire coding unit of the human gene for van Willebrand factor (vWf), a major component of the hemostatic system. The cloned segments span approx. = 175 kilobases of human DNA sequence, and hybridization analysis suggest that the vWf coding unit is approx. =150 kilobases in length. Within one of these clones, the vWF transcription initiation site has been mapped and a portion of the vWf promoter region has been sequenced, revealing a typical TATA box, a downstream CCAAT box, and a perfect downstream repeat of the 8 base pairs containing the transcription start site. Sequencing of a segment of another genomic clone has revealed the vWF translation termination codon. Where tested, comparative restriction analysis of cloned and chromosomal DNA segments strongly suggests that no major alterations occurred during cloning and that there is only one complete copy of the vWf gene in the human haploid genome. Similar analyses of DNA from vWf-producing endothelial cells and nonexpressing leukocytes suggest that vWf gene expression is not accompanied by gross genomic rearrangements. In addition, there is significant homology of C-terminal coding sequences among the vWf genes of several vertebrate species.

• Research Organization: Harvard Medical School, Boston, MA (USA)
• OSTI ID: 5381470
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:13
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENES
DNA-CLONING
HEMATOLOGIC AGENTS
GENETICS
OLIGONUCLEOTIDES
AUTORADIOGRAPHY
HYBRIDIZATION
DNA SEQUENCING
ELECTROPHORESIS
GLUCOPROTEINS
MAN
MESSENGER-RNA
PHOSPHORUS 32
RECOMBINANT DNA
TRANSCRIPTION
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGY
CARBOHYDRATES
CLONING
DAYS LIVING RADIOISOTOPES
DNA
DRUGS
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LIGHT NUCLEI
MAMMALS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
RNA
SACCHARIDES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques