Augmentation of granulocyte/macrophage colony-stimulating factor expression by ultraviolet irradiation is mediated by interleukin 1 in Pam 212 keratinocytes

Journal Article · Mon Jul 01 00:00:00 EDT 1991 · Journal of Investigative Dermatology; (United States)

DOI:https://doi.org/10.1111/1523-1747.ep12477727· OSTI ID:5380758

Nozaki, S; Abrams, J S; Pearce, M K; Sauder, D N ^[1]

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1. Showa Univ., Tokyo (Japan)

Keratinocytes are a potent source of a variety of cytokines including granulocyte-macrophage colony-stimulating factor (GM-CSF). In this study, we have shown that ultraviolet B (UVB) irradiation augments GM-CSF mRNA expression by murine keratinocytes. This is reflected in the increased production of GM-CSF protein by these cells. In the same cell population, exposure to UVB irradiation increases interleukin 1 alpha (IL-1 alpha) mRNA and IL-1 protein as detected by bioactivity. This increase in IL-1 alpha precedes the increase of GM-CSF mRNA. Addition of recombinant IL-1 alpha to the medium increases GM-CSF mRNA expression. Anti-IL-1 alpha antibodies can completely inhibit UV-augmented GM-CSF mRNA expression. These results demonstrate that UVB irradiation-induced augmentation of GM-CSF is mediated by UV-induced IL-1 alpha.

• OSTI ID: 5380758
• Journal Information: Journal of Investigative Dermatology; (United States), Journal Name: Journal of Investigative Dermatology; (United States) Vol. 97:1; ISSN 0022-202X; ISSN JIDEA
• Country of Publication: United States
• Language: English

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