Metabolism of aromatic compounds by actinomycetes: Final report, August 1, 1986-April 1, 1987
The research objectives are: isolate and characterize stable S. viridosporus mutants which are either negative for or enhanced in the ability to produce cellulase, xylanase, and the B-ether cleaving ligninase complex; to isolate and characterize stable mutants which are derepressed for lignin degradation; and clone the gene coding for lignin depolymerization into a nonligninolytic streptomycete, S. lividans. We have obtained stable mutants and an extracellular peroxidase that appears to be a ligninase. A more efficient plasmid vector cloning system for cloning S. viridosporus DNA into S. lividans has been developed. We have successfully used the plasmid to clone a cellulase gene and a peroxidase gene from S. viridosporus into E. coli, and we are now moving the genes into S. lividans to study their expression in this nonligninolytic streptomycete. We have isolated two of the genes coding for enzymes essential for lignocellulose degradation.
- Research Organization:
- Idaho Univ., Moscow (USA). Dept. of Bacteriology and Biochemistry
- DOE Contract Number:
- FG07-86ER13586
- OSTI ID:
- 5380405
- Report Number(s):
- DOE/ER/13586-T1; ON: DE88005858
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ACTINOMYCES
GENETIC ENGINEERING
LIGNIN
BIODEGRADATION
PEROXIDASES
DNA-CLONING
PLASMIDS
DESIGN
BIOLOGICAL PATHWAYS
CELLULASE
MUTANTS
PROGRESS REPORT
XYLANASE
BACTERIA
CARBOHYDRATES
CELL CONSTITUENTS
CHEMICAL REACTIONS
CLONING
DECOMPOSITION
DOCUMENT TYPES
ENZYMES
GLYCOSYL HYDROLASES
HYDROLASES
MICROORGANISMS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
POLYSACCHARIDES
SACCHARIDES
140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989)