Increased intrapulmonary retention of radiolabeled neutrophils in early oxygen toxicity

Journal Article · Mon Feb 01 04:00:00 EST 1988 · Am. Rev. Respir. Dis.; (United States)

DOI:https://doi.org/10.1164/ajrccm/137.2.345· OSTI ID:5380234

Rinaldo, J E; English, D; Levine, J; Stiller, R; Henson, J

Sequential lung injuries, such as oxygen toxicity followed by septicemia, are common during the adult respiratory distress syndrome (ARDS). As these forms of vascular injury may be mediated in part by polymorphonuclear leukocytes (PMN), aberrant interactions between PMN and previously injured pulmonary endothelium are of both theoretical interest and clinical importance. The present study was undertaken to test the hypothesis that early oxygen toxicity at a dose that injuries pulmonary endothelium relatively selectively alters intrapulmonary neutrophil kinetics. Unanesthetized rats breathing 1.0 atmospheres oxygen for 36 h showed ultrastructural endothelial damage but no edema, injury, or neutrophilic inflammation by histologic criteria. However, in these oxygen-toxic animals, whereas initial accumulation of radiolabeled PMN in lungs was normal, washout of PMN was abnormal at 120 min after infusion, at which point the pulmonary retention of radiolabeled PMN in the lungs of oxygen-treated animals was significantly higher than in control animals (139% of control, p less than 0.0096). Features of our methodology, including avoidance of osmotic stress and use of paired control animals, appear to have greatly enhanced the sensitivity of radiolabeled neutrophils for detecting a subtle abnormality of neutrophil-endothelial interactions. Our studies in the oxygen toxicity model provide the first demonstration in vivo of abnormal intrapulmonary neutrophil kinetics in early oxygen toxicity prior to the onset of histologic evidence of lung injury or inflammation.

• Research Organization: Univ. of Pittsburgh School of Medicine, PA (USA)
• OSTI ID: 5380234
• Journal Information: Am. Rev. Respir. Dis.; (United States), Journal Name: Am. Rev. Respir. Dis.; (United States) Vol. 137:2; ISSN ARDSB
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
ANIMALS
ANTIGENS
BACTERIA
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CELL CYCLE
DISEASES
DISTRIBUTION
ELEMENTS
ENDOTHELIUM
ENDOTOXINS
ESCHERICHIA COLI
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LUNGS
MAMMALS
MATERIALS
MICROORGANISMS
NEUTROPHILS
NONMETALS
ORGANIC COMPOUNDS
ORGANOMETALLIC COMPOUNDS
ORGANS
OXYGEN
PATHOGENESIS
PATHOLOGICAL CHANGES
RADIOISOTOPES
RATS
RESPIRATORY SYSTEM
RESPIRATORY SYSTEM DISEASES
RETENTION
RODENTS
SEPTICEMIA
TISSUE DISTRIBUTION
TISSUES
TOXIC MATERIALS
TOXICITY
TOXINS
TRACER TECHNIQUES
VERTEBRATES