Development of nitrogen-fixing monocot-bacteria associations. Final progress report, September 1, 1980-August 31, 1983

Technical Report · Sun Jan 01 04:00:00 EST 1984

OSTI ID:5378623

Brill, W J

Experiments have demonstrated the feasibility of breeding bacteria and cereal plants so that the plant may obtain some of its nitrogen through nitrogen fixation. Corn lines with associative activity were compared to lines without such activity. There is no significant difference between numbers of nitrogen-fixing bacteria on roots of these lines. Azospirillum are found in the extracellular mucilage of the root. Other, yet-to-be-identified bacteria also are found in the mucilage. Techniques, using colloidal gold, have been developed to identify root-associated microbes and to determine which ones contain nitrogenase. Nitrogen fixation seems to require an interaction between an unidentified nitrogen-fixing bacterium and another unidentified bacterium unable to fix nitrogen.

• Research Organization: Wisconsin Univ., Madison (USA)
• DOE Contract Number: AC02-80ER10717
• OSTI ID: 5378623
• Report Number(s): DOE/ER/10717-10; ON: DE84006419
• Country of Publication: United States
• Language: English

Related Subjects

550701 -- Microbiology-- Tracer Techniques
553001* -- Agriculture & Food Technology-- Tracer Techniques-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
ACETYLENE
ALKYNES
ANTIBODIES
BACTERIA
CEREALS
ELECTROPHORESIS
GRAMINEAE
GRASS
HYBRIDIZATION
HYDROCARBONS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAIZE
MICROORGANISMS
MONOCLONAL ANTIBODIES
NITROGEN 15
NITROGEN FIXATION
NITROGEN ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PLANTS
STABLE ISOTOPES
TRACER TECHNIQUES