Action spectra determined with tunable dye laser light for light-induced proton efflux and uptake in membrane vesicles of Halobacterium halobium
Journal Article
·
· J. Biol. Chem.; (United States)
OSTI ID:5375224
- Cornell Univ., Ithaca, NY
A new pigment from Halobacterium halobium has been previously described which mediates the electrogenic extrusion of sodium ions when illuminated. Using essentially monochromatic light (0.05 nm bandwidth), maximum activity for this pigment occurred at 590 nm as monitored by passive proton uptake in envelope vesicles. The uncoupler bis(hexafluoroacetonyl)-acetone did not shift peak activity, but did increase passive proton influx. When the new pigment (NaP/sub 590/) and bacteriorhodopsin, the well characterized proton pump, were compared at their respective wavelengths of maximum activity, NaP/sub 590/ required approximately 100-fold more energy to achieve saturation.
- OSTI ID:
- 5375224
- Journal Information:
- J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 255:8; ISSN JBCHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200* -- Biochemistry
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
CHARGED-PARTICLE TRANSPORT
CHEMICAL REACTIONS
CHEMISTRY
COMPARATIVE EVALUATIONS
ELECTROMAGNETIC RADIATION
KINETICS
MEMBRANES
MICROORGANISMS
MONOCHROMATIC RADIATION
PHOTOCHEMICAL REACTIONS
PHOTOCHEMISTRY
PIGMENTS
PROTON TRANSPORT
RADIATION TRANSPORT
RADIATIONS
REACTION KINETICS
SPECTRA
VISIBLE RADIATION
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
CHARGED-PARTICLE TRANSPORT
CHEMICAL REACTIONS
CHEMISTRY
COMPARATIVE EVALUATIONS
ELECTROMAGNETIC RADIATION
KINETICS
MEMBRANES
MICROORGANISMS
MONOCHROMATIC RADIATION
PHOTOCHEMICAL REACTIONS
PHOTOCHEMISTRY
PIGMENTS
PROTON TRANSPORT
RADIATION TRANSPORT
RADIATIONS
REACTION KINETICS
SPECTRA
VISIBLE RADIATION