Effects of permeant buffers on the initial time course of photophosphorylation and postillumination phosphorylation

Journal Article · Tue Mar 25 00:00:00 EST 1980 · J. Biol. Chem.; (United States)

OSTI ID:5375167

Vinkler, C; Avron, M; Boyer, P D

Permeant buffers (pyridine, imidazole, or phosphate) caused similar increases in the time required for onset of ATP synthesis in the light in the presence of valinomycin and K+; and in the illumination time required for postillumination phosphorylation with or without valinomycin and K+. Based on prior evidence, the minimum illumination time required for postillumination phosphorylation in thylakoid membranes is taken as a measure of the time required for formation of a transmembrane pH gradient sufficient to drive ATP synthesis. Our results are consistent with the view that, following illumination, as the transient transmembrane electric gradient decays, the establishment and maintenance of a pH gradient serve for energy transfer from the photosystems to the ATP synthase complex.

• Research Organization: Univ. of California, Los Angeles
• OSTI ID: 5375167
• Journal Information: J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 255:6; ISSN JBCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400500 -- Photochemistry
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
ANTIBIOTICS
ATP
AZINES
AZOLES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CATIONS
CELL CONSTITUENTS
CELL MEMBRANES
CHARGED PARTICLES
CHEMICAL REACTIONS
CHEMISTRY
CHLOROPLASTS
DRUGS
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
IONS
KINETICS
MEMBRANES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PH VALUE
PHOSPHATES
PHOSPHORUS COMPOUNDS
PHOSPHORYLATION
PHOTOCHEMICAL REACTIONS
PHOTOCHEMISTRY
POTASSIUM COMPOUNDS
PYRIDINE
PYRIDINES
REACTION KINETICS
SYNTHESIS
VALINOMYCIN