Calcium mobilization by cadmium or decreasing extracellular Na sup + or pH in coronary endothelial cells

Journal Article · Tue Jan 01 04:00:00 EST 1991 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(91)90152-K· OSTI ID:5374521

Dwyer, S D; Zhuang, Yingxin; Smith, J B ^[1]

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1. Univ. of Alabama, Birmingham (United States)

Replacing extracellular Na{sup +} with choline transiently increased cytoplasmic free Ca{sup 2+} ((Ca{sup 2+}){sub i}) more than 5-fold in coronary endothelial cells. Removing external Na{sup +} stimulated {sup 45}Ca{sup 2+} efflux {approximately}4-fold and influx {approximately}1.7-fold. The stimulation of efflux was independent of extracellular Ca{sup 2+} and the osmotic Na{sup +} substitute. The release of stored Ca{sup 2+}, rather than Ca{sup 2+} influx via Na{sup +}-Ca{sup 2+} exchange, probably causes the increase in (Ca{sup 2+}){sub i} and {sup 45}Ca{sup 2+} efflux. Cadmium or decreasing external, not intracellular, pH transiently increased (Ca{sup 2+}){sub i}. Cd{sup 2+} and some other divalent metals also stimulated {sup 45}Ca{sup 2+} efflux. The potency order of the metals that stimulated efflux was Cd{sup 2+} > Co{sup 2+} > Ni{sup 2+} > Fe{sup 2+} > Mn{sup 2+}. Incubating the cells with Zn{sup 2+} prior to assaying efflux in the absence of Zn{sup 2+} strongly inhibited the stimulation of {sup 45}Ca{sup 2+} efflux by Cd{sup 2+}, pH 6, and the removal of external Na{sup +} or pH trigger the release of stored Ca{sup 2+} by stimulating a cell surface receptor.'

• OSTI ID: 5374521
• Journal Information: Experimental Cell Research; (United States), Journal Name: Experimental Cell Research; (United States) Vol. 192:1; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

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560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
AMINES
AMMONIUM COMPOUNDS
ANIMAL TISSUES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CADMIUM COMPOUNDS
CALCIUM 45
CALCIUM CHLORIDES
CALCIUM COMPOUNDS
CALCIUM HALIDES
CALCIUM ISOTOPES
CHLORIDES
CHLORINE COMPOUNDS
CHOLINE
COBALT COMPOUNDS
DAYS LIVING RADIOISOTOPES
DRUGS
ELEMENTS
ENDOTHELIUM
EVEN-ODD NUCLEI
HALIDES
HALOGEN COMPOUNDS
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IRON COMPOUNDS
ISOTOPES
LIPOTROPIC FACTORS
MANGANESE COMPOUNDS
MEMBRANE PROTEINS
MEMBRANE TRANSPORT
METALS
NICKEL COMPOUNDS
NUCLEI
ORGANIC COMPOUNDS
PH VALUE
PROTEINS
QUATERNARY COMPOUNDS
RADIOISOTOPES
RECEPTORS
TISSUES
TRANSITION ELEMENT COMPOUNDS
ZINC COMPOUNDS