/sup 13/C NMR evidence of the slow exchange of tryptophans in dihydrofolate reductase between stable conformations
Journal Article
·
· Biochem. Biophys. Res. Commun.; (United States)
- Univ. of California, Los Alamos, NM
/sup 13/C NMR spectra are reported for dihydrofolate reductase of Streptococcus faecium labeled with (..gamma..-/sup 13/C)tryptophan. Two of the four tryptophans generate unusual resonances indicating slow exchange of the residues between alternative stable conformations. Since 3', 5'-dichloromethotrexate sharpens two of the resonances, it apparently locks the corresponding residues into one conformation.
- OSTI ID:
- 5374346
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 86:3; ISSN BBRCA
- Country of Publication:
- United States
- Language:
- English
Similar Records
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/sup 13/C NMR studies of bacterial dihydrofolate reductase containing (methyl-/sup 13/C)methionine and (guanido-/sup 13/C)arginine. [Streptococcus]
Nuclear magnetic resonance study of interaction of ligands with Streptococcus faecium dihydrofolate reductase labeled with (. gamma. -/sup 13/C)tryptophan
Journal Article
·
Fri Dec 31 23:00:00 EST 1976
· Biochem. Biophys. Res. Commun.; (United States)
·
OSTI ID:6389745
/sup 13/C NMR studies of bacterial dihydrofolate reductase containing (methyl-/sup 13/C)methionine and (guanido-/sup 13/C)arginine. [Streptococcus]
Conference
·
Sat Dec 31 23:00:00 EST 1977
·
OSTI ID:6744095
Nuclear magnetic resonance study of interaction of ligands with Streptococcus faecium dihydrofolate reductase labeled with (. gamma. -/sup 13/C)tryptophan
Journal Article
·
Thu Dec 31 23:00:00 EST 1981
· Biochemistry; (United States)
·
OSTI ID:6299731
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AZOLES
BACTERIA
BIOCHEMICAL REACTION KINETICS
CARBON 13
CARBON ISOTOPES
CARBOXYLIC ACIDS
DYNAMIC FUNCTION STUDIES
ENZYMES
EVEN-ODD NUCLEI
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INDOLES
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIGHT NUCLEI
MAGNETIC RESONANCE
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PYRROLES
REACTION KINETICS
RESONANCE
STABLE ISOTOPES
STREPTOCOCCUS
STRUCTURAL CHEMICAL ANALYSIS
TRYPTOPHAN
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AZOLES
BACTERIA
BIOCHEMICAL REACTION KINETICS
CARBON 13
CARBON ISOTOPES
CARBOXYLIC ACIDS
DYNAMIC FUNCTION STUDIES
ENZYMES
EVEN-ODD NUCLEI
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INDOLES
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIGHT NUCLEI
MAGNETIC RESONANCE
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PYRROLES
REACTION KINETICS
RESONANCE
STABLE ISOTOPES
STREPTOCOCCUS
STRUCTURAL CHEMICAL ANALYSIS
TRYPTOPHAN