Isolated photochemical reaction centers from bacteriochlorophyll b - containing organisms

Conference · Fri Dec 31 23:00:00 EST 1976

OSTI ID:5373423

Thornber, J P; Dutton, P L; Fajer, J; Parson, W W; Prince, R C; Tiede, D M; Windsor, M W

Optical, ESR and pulsed laser spectroscopic examination of isolated bacteriochlorophyll (BChl) b-containing reaction centers (RCs) demonstrates that their composition (1 P960 : 2 bacteriopheophytin (BPh) b : 2 cyt c 558 : 2 cyt c 553 : BChl/BPh = 2) and their mechanism for charge separation are very similar to those in BChl a-containing RCs; however, P960/sup +/, the oxidized form of the primary electron donor, is apparently not a symmetrical dimer of BChl b. The ''primary'' electron acceptor (X) is a quinone-iron complex, and I, the intermediary electron carrier between P960 and X, is BPh which interact(s) strongly with one or more of the other components in the RC. Comparison of the optical spectra of isolated RCs of the BChl b-containing bacteria, Rhodopseudomonas viridis and Thiocapsa pfennigii, with those of their intact cells shows that the BPh in the RC is not generated during the isolation procedure.

• Research Organization: Brookhaven National Lab., Upton, N.Y. (USA); Pennsylvania State Univ., University Park (USA). Dept. of Chemistry; Washington State Univ., Pullman (USA). Dept. of Chemistry; Washington Univ., Seattle (USA). Dept. of Biochemistry
• DOE Contract Number: EY-76-C-02-0016
• OSTI ID: 5373423
• Report Number(s): BNL-23306; CONF-770951-3
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIOCHEMICAL REACTION KINETICS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHLOROPHYLL
ELECTRONS
ELEMENTARY PARTICLES
FERMIONS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
KINETICS
LEPTONS
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOTOCHEMICAL REACTIONS
PHYTOCHROMES
PIGMENTS
PORPHYRINS
REACTION KINETICS
RHODOPSEUDOMONAS
SPECTRA
SPECTROSCOPY