Turnover of endogeneous microinjected and sequestered protein in xenopus oocytes

Journal Article · Mon Jan 01 04:00:00 EST 1979 · Exp. Cell Res.; (United States)

DOI:https://doi.org/10.1016/0014-4827(79)90355-0· OSTI ID:5372855

Wallace, R A ^[1]; Hollinger, T G

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1. Oak Ridge National Lab., TN

Pulse-labeled oocyte proteins were found to be a maximum average half-life of 73 h. In general, larger peptides underwent degradation at a faster rate than smaller peptides. In this respect, oocytes are similar to most other cells. Microinjected /sup 125/I-labeled bovine serum albumin (BSA) was degraded over a 40 h period with a half-life of 20 to 30 h, regardless of the method of protein labeling, culture medium employed, size of oocyte microinjected, or hormonal history of the oocyte. The last two results, if applicable to oocyte proteins in general, imply that protein catabolism is constant throughout the later stages of oogenesis and that growth is primarily regulated by a stimulation of anabolism. Individual proteins microinjected into oocytes undergo rates of degradation consistent with turnover rates obtained in other systems. Sequestered /sup 125/I-labeled BSA is only partially (40%) degraded, which indicates that, unlike microinjected /sup 125/I-labeled BSA, it has access to a cytoplasmic compartment (yolk platelets.) within which it is relatively stable.

• OSTI ID: 5372855
• Journal Information: Exp. Cell Res.; (United States), Journal Name: Exp. Cell Res.; (United States) Vol. 119; ISSN ECREA
• Country of Publication: United States
• Language: English

Related Subjects

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550300* -- Cytology
550500 -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
AMPHIBIANS
ANABOLISM
ANIMALS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CATABOLISM
CELL CULTURES
CELL PROLIFERATION
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GAMETOGENESIS
GERM CELLS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
METABOLISM
NUCLEI
ODD-EVEN NUCLEI
OOCYTES
OOGENESIS
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
TRACER TECHNIQUES
VERTEBRATES