Effect of zinc on the interaction between fibrinogen and platelet glycoprotein IIb/IIIa (GPIIb/IIIa)

Conference · Sun Mar 10 23:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5372503

Trybulec, M; Cook, J J; Niewiarowski, S ^[1]

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1. Temple Univ., Philadelphia, PA (United States)

In the present study, the authors tested the effect of Zn++ on the interaction between fibrinogen (Fg) and its platelet membrane receptor in three different systems. (1) Fg binding to ADP-stimulated platelets showed a pronounced elevation in the presence of 1 mM zinc. Scatchard analysis of this data revealed a significant increase in the number of Fg binding sites, however, binding affinity was not changed. This increase was only partially blocked by a GPIIb/IIIa complex-specific monoclonal antibody (A{sub 2}A{sub 9}). (2) Zn++ enhanced Fg-induced aggregation of chymotrypsin-treated platelets. (3) Zn++ but not Mn++ or MG++, increased binding of purified GPIIb/IIIa to Fg immobilized on the ELISA plate in the presence of 0.5 mM calcium. The same extent of binding occurred even in the absence of calcium provided that Zn++ was present. A{sub 2}A{sub 9} antibody only partially blocked this reaction. The authors propose that zinc-induced changes in the structure of GPIIb/IIIa complex make the receptor more available to fibrinogen.

• OSTI ID: 5372503
• Report Number(s): CONF-9104107--
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Vol. 5:4; ISSN 0892-6638; ISSN FAJOE
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTIBODIES
BIOASSAY
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD COAGULATION FACTORS
BLOOD PLATELETS
BODY FLUIDS
CELL CONSTITUENTS
CELL MEMBRANES
COAGULANTS
DRUGS
ELEMENTS
ENZYME IMMUNOASSAY
FIBRINOGEN
GLOBULINS
HEMATOLOGIC AGENTS
HEMOSTATICS
IMMUNOASSAY
KINETICS
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
METALS
MONOCLONAL ANTIBODIES
MORPHOLOGICAL CHANGES
ORGANIC COMPOUNDS
PROTEINS
REACTION KINETICS
RECEPTORS
ULTRASTRUCTURAL CHANGES
ZINC