Characteristics of Anabaena variabilis influencing plaque formation by cyanophage N-1

Journal Article · Sun Jul 01 04:00:00 EDT 1979 · J. Bacteriol.; (United States)

OSTI ID:5372167

Currier, T C; Wolk, C P

Phage N-1 grown in Anabaena strain 7120 (N-1 . 7120) forms plaques on A. variabilis about 10/sup -7/ to 10/sup -6/ as efficiently as on Anabaena 7120. By manipulating different characteristics of the interaction between phage and host, it was possible to increase the relative efficiency of plaque formation to 0.38. Growth of A. variabilis at 40/sup 0/C for at least three generations resulted in an increase in the rate of phage adsorption and a 10-fold increase in the efficiency of plaque formation. The efficiency of plaque formation was further increased about 42-fold, with little or no further increase in rate of adsorption, in a variant strain, A. variabilis strain FD, isolated from a culture of A. variabilis which had grown for more than 30 generations at 40/sup 0/C. The low relative efficiency of plaque formation by N-1 . 7120 on A. variabilis could be partially accounted for if A. variabilis contains a deoxyribonucleic acid restriction endonuclease which is absent from Anabaena 7120. Indirect evidence for such an endonuclease included the following: (i) phage N-1 grown in A. variabilis (N-1 . Av) had approximately a 7 x 10/sup 3/-fold higher relative efficiency of plaque formation on A. variabilis than had N-1 . 7120; and (ii) the efficiency of plaque formation by N-1 . 7120 on A. variabilis strain FD was increased by up to 146-fold after heating the latter organism at 51/sup 0/C.

• Research Organization: Michigan State Univ., Lansing
• OSTI ID: 5372167
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 139:1; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

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550400 -- Genetics
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BACTERIOPHAGES
BIOCHEMICAL REACTION KINETICS
CFU
COLONY FORMATION
DNA
ENZYMES
GENES
HOST
KINETICS
MICROORGANISMS
NUCLEASES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PHOSPHOTRANSFERASES
REACTION KINETICS
TEMPERATURE EFFECTS
TRANSFERASES
VIRUSES